Two-dimensional differential in gel electrophoresis (2D-DIGE) analysis of grape berry proteome during postharvest withering

TitoloTwo-dimensional differential in gel electrophoresis (2D-DIGE) analysis of grape berry proteome during postharvest withering
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2011
AutoriDi Carli, Mariasole, Zamboni A., Pè M.E., Pezzotti M., Lilley K.S., Benvenuto Eugenio, and Desiderio Angiola
RivistaJournal of Proteome Research
Paginazione429 - 446
Data di pubblicazione2011
ISBN Number15353893 (ISSN)
Parole chiave2D-DIGE, Amino Acid Sequence, article, berry, Berry proteome, Electrophoresis, food quality, fruit ripening, Gel, gel electrophoresis, Germplasm, grape, Mass Spectrometry, Metabolic Networks and Pathways, Molecular Sequence Data, Multivariate analysis, Odors, Plant development, Plant extracts, Plant protein extraction, Plant Proteins, postharvest period, postharvest withering, priority journal, protein analysis, protein metabolism, protein processing, protein synthesis, proteome, Proteomics, stress, total quality management, two dimensional differential in gel electrophoresis, Two-Dimensional, Vitaceae, Vitis, Vitis vinifera, Vitis vinifera extract, Wine, Withering

The practice of postharvest withering is commonly used to correct quality traits and sugar concentration of high quality wines. To date, changes in the metabolome during the berry maturation process have been well documented; however, the biological events which occur at the protein level have yet to be fully investigated. To gain insight into the postharvest withering process, we studied the protein expression profiles of grape (Corvina variety) berry development focusing on withering utilizing a two-dimensional differential in gel electrophoresis (2D-DIGE) proteomics approach. Comparative analysis revealed changes in the abundance of numerous soluble proteins during the maturation and withering processes. On a total of 870 detected spots, 90 proteins were differentially expressed during berry ripening/withering and 72 were identified by MS/MS analysis. The majority of these proteins were related to stress and defense activity (30%), energy and primary metabolism (25%), cytoskeleton remodelling (7%), and secondary metabolism (5%). Moreover, this study demonstrates an active modulation of metabolic pathways throughout the slow dehydration process, including de novo protein synthesis in response to the stress condition and further evolution of physiological processes originated during ripening. These data represent an important insight into the withering process in terms of both Vitis germplasm characterization and knowledge which can assist quality improvement. © 2011 American Chemical Society.


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