Comparison of drought stress response and gene expression between a GM maize variety and a near-isogenic non-GM variety

TitoloComparison of drought stress response and gene expression between a GM maize variety and a near-isogenic non-GM variety
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2015
AutoriGullì, M., Salvatori E., Fusaro L., Pellacani C., Manes F., and Marmiroli N.
RivistaPLoS ONE
Volume10
ISSN19326203
Parole chiavearticle, controlled study, CrylAb gene, drought, drought stress, Droughts, gene activation, Gene expression, gene expression profiling, gene expression regulation, gene induction, genetic transcription, Genetically Modified, genetics, Maize, near isogenic line, nonhuman, photosynthesis, Plant, plant gene, plant growth, Plant response, Plants, procedures, transgene, transgenic plant, upregulation, varietas, water deficit, Zea mays
Abstract

Maize MON810, grown and commercialised worldwide, is the only cultivated GM event in the EU. Maize MON810, variety DKC6575, and the corresponding near-isogenic line Tietar were studied in different growth conditions, to compare their behaviour in response to drought. Main photosynthetic parameters were significantly affected by drought stress in both GM and non-GM varieties to a similar extent. Though DKC6575 (GM) had a greater sensitivity in the early phase of stress response as compared with Tietar (non-GM), after six days of stress they behaved similarly, and both varieties recovered from stress damage. Profiling gene expression in water deficit regimes and in a generalised drought stress condition showed an up-regulation of many stress-responsive genes, but a greater number of differentially expressed genes was observed in Tietar, with genes belonging to transcription factor families and genes encoding heat shock proteins, late embryogenesis abundant proteins and detoxification enzymes. Since induction of these genes have been indicated from the literature as typical of stress responses, their activation in Tietar rather than in DKC6575 may be reminiscent of a more efficient response to drought. DKC6575 was also analysed for the expression of the transgene CryIAb (encoding the delta-endotoxin insecticidal protein) in water deficit conditions. In all the experiments, the CryIAb transcript was not influenced by drought stress, but was expressed at a constant level. This suggests that though possessing a different pattern of sensitivity to stress, the GM variety maintains the same expression level for the transgene. © 2015 Gullì et al.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84923383696&doi=10.1371%2fjournal.pone.0117073&partnerID=40&md5=2f3c24270d1d80439e424a80521aa267
DOI10.1371/journal.pone.0117073