This paper introduces first results of characterization of medullobastoma cell lines using high frequency dielectrophoresis (HF-DEP) approach at frequencies ranging up to few hundreds of MHz. Such technique enables accessing intracellular dielectric property specificities on individual cells. Actually, the frequency dependent DEP behavior of several medullobastoma cells has been studied showing clear differences between cells from different types which correlate well the biological specificity established though cell phenotype characterization. These results should pave the way to novel and complementary cell discrimination approach based on a label free HF-DEP crossover frequency signature measurement. © 2018 FESB, University of Split.