Production of magnetic modified microalgae using iron oxide nanoparticles and electroporation technique

TitoloProduction of magnetic modified microalgae using iron oxide nanoparticles and electroporation technique
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2019
AutoriSavvidou, M.G., Banis G., Ferraro A., Molino Antonio, Karatza D., Chianese S., Musmarra D., Kolisis F., and Hristoforou E.
RivistaChemical Engineering Transactions
Volume73
Paginazione13-18
ISSN22839216
Astratto

Immobilization of living cells is a useful technique that allows to enhance the biotechnological properties of many organisms. Currently, cell-immobilization is obtained via carrier adsorption, self-aggregation and entrapment. We are currently investigating the possibility to immobilize microalgae cells using magnetic nanoparticles on a magnetic surface. The principle is based on the introduction, inside the cell-body of microalgae, superparamagnetic nanoparticles through electroporation. Once microalgae have absorbed enough magnetic nanoparticles they will be responding to an external magnetic field. By using a flat magnetic surface, it will be possible to trap them in a specific position by means of magnetic force. Importantly, immobilized cells will be covered with a thin layer of water and during cell division only one of the daughter cells will keep the magnetic nanoparticles since they will face permanently the magnetic surface. The first step towards this goal is the introduction of magnetic nanoparticles in the microalgae cells. This work will present preliminary results of magnetic cells preparation. Magnetic nanoparticles covered with lipid, to make them biocompatible, and electroporation method to inset nanoparticles in the cells have been used. The protocol to obtain magnetic cells and viability assay that proved the feasibility of the method will be described. Copyright © 2019, AIDIC Servizi S.r.l.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85066973405&doi=10.3303%2fCET1973003&partnerID=40&md5=a1fb8db0f3416df8f7674a0a4a762876
DOI10.3303/CET1973003