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Flow Sorting and Molecular Cytogenetic Identification of Individual Chromosomes of Dasypyrum villosum L. (H. villosa) by a Single DNA Probe

TitoloFlow Sorting and Molecular Cytogenetic Identification of Individual Chromosomes of Dasypyrum villosum L. (H. villosa) by a Single DNA Probe
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2012
AutoriGrosso, V., Farina Anna, Gennaro A., Giorgi Debora, and Lucretti Sergio
RivistaPLoS ONE
Volume7
ISSN19326203
Parole chiavearticle, chromosome 6, chromosome 6v, chromosome identification, chromosome segregation, chromosome structure, Chromosomes, controlled study, crop improvement, Dasypyrum villosum, DNA, DNA content, DNA probe, DNA Probes, Flow cytometry, fluorescence, fluorescence in situ hybridization, Genetic, genetic marker, Genetic resistance, genetic trait, Genetic variability, Genome, Haynaldia villosa, heterozygote, hybridization, In Situ Hybridization, Karyotype, Karyotyping, Microsatellite Repeats, molecular cloning, molecular mechanics, nonhuman, Plant, Plant breeding, plant chromosome, Ploidies, Poaceae, seed storage, simple sequence repeat, Triticum, Triticum aestivum, wheat
Abstract

Dasypyrum villosum (L.) Candargy (sin. Haynaldia villosa) is an annual wild diploid grass species (2n = 2x = 14; genome VV) belonging to the Poaceae family, which is considered to be an important source of biotic and abiotic stress resistance genes for wheat breeding. Enhanced characterization of D. villosum chromosomes can facilitate exploitation of its gene pool and its use in wheat breeding programs. Here we present the cytogenetic identification of D. villosum chromosomes on slide by fluorescent in situ hybridization (FISH), with the GAA simple sequence repeat (SSR) as a probe. We also describe the isolation and the flow cytometric analysis of D. villosum chromosomes in suspension, resulting in a distinguished flow karyotype. Chromosomes were flow sorted into three fractions, according their DNA content, one of which was composed of a single type of chromosome, namely 6 V, sorted with over 85% purity. Chromosome 6 V is known to carry genes to code for important resistance and seed storage characteristics, and its isolation represents a new source of genetic traits and specific markers useful for wheat improvement. © 2012 Grosso et al.

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cited By 14

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84869805521&doi=10.1371%2fjournal.pone.0050151&partnerID=40&md5=b716b305971c88bf8f4ad89360068cb9
DOI10.1371/journal.pone.0050151
Citation KeyGrosso2012