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S-Nitrosoglutathione Reductase Plays Opposite Roles in SH-SY5Y Models of Parkinson's Disease and Amyotrophic Lateral Sclerosis.

TitoloS-Nitrosoglutathione Reductase Plays Opposite Roles in SH-SY5Y Models of Parkinson's Disease and Amyotrophic Lateral Sclerosis.
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2015
AutoriRizza, Salvatore, Cirotti Claudia, Montagna Costanza, Cardaci Simone, Consales Claudia, Cozzolino Mauro, Carrì Maria Teresa, Cecconi Francesco, and Filomeni Giuseppe
RivistaMediators Inflamm
Volume2015
Paginazione536238
Data di pubblicazione2015
ISSN14661861
Parole chiaveAldehyde Oxidoreductases, amyotrophic lateral sclerosis, Animals, Brain, Cell Line, Tumor, Cell Survival, Female, gene silencing, Humans, male, Membrane Potentials, Mice, Mice, Inbred C57BL, Mice, Knockout, Microscopy, Fluorescence, Mitochondria, Neurodegenerative Diseases, Neurons, NF-E2-Related Factor 2, Oxidative stress, Parkinson disease, Phenotype, RNA, Small Interfering, Spinal Cord
Abstract

Oxidative and nitrosative stresses have been reported as detrimental phenomena concurring to the onset of several neurodegenerative diseases. Here we reported that the ectopic modulation of the denitrosylating enzyme S-nitrosoglutathione reductase (GSNOR) differently impinges on the phenotype of two SH-SY5Y-based in vitro models of neurodegeneration, namely, Parkinson's disease (PD) and familial amyotrophic lateral sclerosis (fALS). In particular, we provide evidence that GSNOR-knocking down protects SH-SY5Y against PD toxins, while, by contrast, its upregulation is required for G93A-SOD1 expressing cells resistance to NO-releasing drugs. Although completely opposite, both conditions are characterized by Nrf2 localization in the nuclear compartment: in the first case induced by GSNOR silencing, while in the second one underlying the antinitrosative response. Overall, our results demonstrate that GSNOR expression has different effect on neuronal viability in dependence on the stimulus applied and suggest that GSNOR could be a responsive gene downstream of Nrf2 activation.

DOI10.1155/2015/536238
Alternate JournalMediators Inflamm.
Citation Key6754
PubMed ID26491229
PubMed Central IDPMC4600557