|Titolo||Influence of a low background radiation environment on biochemical and biological responses in V79 cells|
|Tipo di pubblicazione||Articolo su Rivista peer-reviewed|
|Anno di Pubblicazione||2002|
|Autori||Satta, L., Antonelli Francesca, Belli M., Sapora O., Simone G., Sorrentino E., Tabocchini M.A., Amicarelli F., Ara C., Cerù M.P., Colafarina S., Devirgiliis L.C., De Marco Alessandra, Balata M., Falgiani A., and Nisi S.|
|Rivista||Radiation and Environmental Biophysics|
|Parole chiave||Air pollution, animal cell, Animalia, Animals, Apoptosis, article, Background Radiation, Cell Division, cell growth, Cell Line, Cell Survival, cesium 137, controlled study, Cricetinae, Cricetulus griseus, cycloheximide, Dose-Response Relationship, environmental exposure, enzyme assay, Fibroblasts, gamma radiation, Gamma rays, Gene expression, gene mutation, Glutathione peroxidase, Glutathione Transferase, hamster, Indoor, Ionizing radiation, lung, mammal cell, Mammalia, mutation, nonhuman, oncogene c myc, protein p53, Proto-Oncogene Proteins c-myc, Radiation, Radiation Dosage, radiation dose, Radioactive, Radon, Reproducibility of Results, Sensitivity and Specificity, thermoluminescence dosimeter, tumor suppressor gene, Tumor Suppressor Protein p53|
We present the results of an experiment aimed at comparing the effects of different background radiation environments on metabolism and responses to γ-rays and cycloheximide of cultured mammalian cells. Chinese hamster V79 cells were maintained in exponential growth in parallel for up to 9 months at the Istituto Superiore di Sanità (ISS) and at the INFN-Gran Sasso underground Laboratory (LNGS) where exposure due to γ-rays and to radon was reduced by factors of about 70 and 25, respectively. After 9 months the cells grown at the LNGS (cumulative γ dose about 30 μGy, average radon concentration around 5 Bq/m3), compared to the cells grown at the ISS (cumulative γ-ray dose about 2 mGy, average radon concentration around 120 Bq/m3), exhibited i) a significant increase of the cell density at confluence, ii) a significantly higher capacity to scavenge organic and inorganic hydroperoxides but a reduced scavenging capacity towards superoxide anions and iii) an increase in both the basal hprt mutation frequency and sensitivity to the mutagenic effect of γ-rays. The cells grown at the LNGS also showed a greater apoptotic sensitivity starting at the third month of culture, that was no longer detected after 9 months. Overall, these data suggest a role of background ionizing radiation in determining an adaptive response, although they cannot be considered conclusive.
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