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Enzyme/semiconductor nanoclusters combined systems for novel amperometric biosensors

TitoloEnzyme/semiconductor nanoclusters combined systems for novel amperometric biosensors
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2005
AutoriVastarella, W, and Nicastri R.
RivistaTalanta
Volume66
Paginazione627-633
Parole chiaveAmperometric biosensors, Amperometric sensors, Biosensors, CdS nanoparticles, Electrochemistry, Electrodes, Enzyme-semiconductor hybrids, Enzymes, Formaldehyde dehydrogenase, liquid chromatography, Nanostructured materials, oxidation, Semiconductor materials
Abstract

In this work quantum-sized CdS nanocrystals were synthesized using a quaternary water-in-oil microemulsion and immobilized onto gold working electrode by self-assembled monolayers techniques. Formaldehyde dehydrogenase was covalently immobilized onto a protecting membrane, which was stratified on part of the semiconductor nanoparticles modified electrode. The covalent enzyme immobilization has been required to improve the stability of the catalytic oxidation of formaldehyde, which occurs after light stimulation of the semiconductor through the electron/hole recombination. A study about the best electrochemical oxidation potentials under different flow conditions was performed. Preliminary sensor stability and interferences tests were also carried out, for a sensitive and selective detection of formaldehyde. A detection limit of 41 ppb of formaldehyde was calculated and an operational stability of 6 h was achieved under flow conditions by means of this novel amperometric biosensor based on FDH-semiconductor hybrid systems, not requiring NAD+/NADH as charge transfer in the enzymatic reaction. © 2004 Elsevier B.V. All rights reserved.

Note

cited By 79

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-18844382891&doi=10.1016%2fj.talanta.2004.12.007&partnerID=40&md5=2a4a9028296b6283c3db439fdf948d4b
DOI10.1016/j.talanta.2004.12.007
Citation KeyVastarella2005627