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Lonidamine transiently affects spermatogenesis in pubertal CD1 mice.

TitoloLonidamine transiently affects spermatogenesis in pubertal CD1 mice.
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2005
AutoriTraina, Maria Elsa, Guarino Maria, Urbani Elisabetta, Saso Luciano, Eleuteri Patrizia, Cordelli Eugenia, Rescia Michele, Leter Giorgio, and Spanò M.
RivistaContraception
Volume72
Issue4
Paginazione262-7
Data di pubblicazione2005 Oct
ISSN00107824
Parole chiaveAnimals, Antispermatogenic Agents, chromatin, DNA, Flow cytometry, Indazoles, male, Mice, Organ Size, sexual maturation, Sperm Count, Sperm Head, spermatogenesis, Spermatozoa, testis
Abstract

Lonidamine (LND) [1-(2,4-dichlorobenzyl)-1H-indazole-3-carboxylic acid], a well-known antispermatogenic drug, was studied for the first time in pubertal mice to assess its possible effects on spermatogenesis. Male CD1 mice were orally treated on Postnatal Day (PND) 28 with a single dose of LND (100 mg/kg body weight) and sacrificed on PND30, PND42, PND74 and PND123. On PND30 (48 h after dosing), severe testicular effects were evidenced in the treated animals: (a) reduction of the testicular sperm head concentration (approximately 50% of the control value); (b) changes in the spermatogenic cell type distribution (mild decrease of the elongated spermatids and S-phase cells fractions); and (c) morphological alterations of the Sertoli cell cytoplasm and germ cell exfoliation. These changes were recovered in adulthood, on PND74 and PND123. However, no effect on sperm chromatin structure was detected on the epididymal sperm of mature mice by sperm chromatin structure assay, suggesting that LND did not interfere with the process of chromatin reorganization and DNA packaging.

Note

cited By 8

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-25144448032&doi=10.1016%2fj.contraception.2005.04.008&partnerID=40&md5=fb98b06044ca5466f3fd726d51ce2c84
DOI10.1016/j.contraception.2005.04.008
Alternate JournalContraception
Citation Key4921
PubMed ID16181969