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Diepoxybutane cytotoxicity on mouse germ cells is enhanced by in vivo glutathione depletion: A flow cytometric approach

TitoloDiepoxybutane cytotoxicity on mouse germ cells is enhanced by in vivo glutathione depletion: A flow cytometric approach
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione1998
AutoriSpanò, M., Cordelli Eugenia, Leter Giorgio, and Pacchierotti Francesca
RivistaMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume397
Paginazione37-43
ISSN00275107
Parole chiaveanimal cell, Animalia, Animals, article, butadiene diepoxide, Butadienes, Cell cycle, cell differentiation, Cytotoxicity, DNA, drug effect, Environmental Pollution, enzyme activation, Epoxy Compounds, Flow cytometry, Fluorescent Dyes, Germ Cells, glutathione, glutathione metabolism, Inbred Strains, Indoles, Ketones, male, Mice, mouse, Mutagens, nonhuman, Organ Size, Ploidies, priority journal, Spermatids, spermatogenesis
Abstract

Diepoxybutane is one of the key metabolites of butadiene, a compound of high environmental and occupational concern. The effects of diepoxybutane on mouse reproductive cells have been previously characterized by flow cytometry demonstrating a specific, dose-dependent cytotoxicity for differentiating spermatogonia. It is known that butadiene epoxides, deriving from butadiene bioactivation by cytochrome P450-monooxygenase systems, can be enzymatically conjugated to glutathione by glutathione S-transferases. In this paper, we tested the hypothesis whether a pretreatment with phorone, a well-known intracellular glutathione depleter, would enhance the germ cell cytotoxicity of diepoxybutane. Results were consistent with an active role played in vivo by the glutathione-detoxifying system, as diepoxybutane cytotoxicity was increased after chemically induced reduction of glutathione concentration.

Note

cited By 20

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0032536126&doi=10.1016%2fS0027-5107%2897%2900193-0&partnerID=40&md5=a8d8060a1df7c0cf6f57d0f030407d85
DOI10.1016/S0027-5107(97)00193-0
Citation KeySpanò199837