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A multi-omic Nicotiana benthamiana resource for fundamental research and biotechnology

TitoloA multi-omic Nicotiana benthamiana resource for fundamental research and biotechnology
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2023
AutoriRanawaka, Buddhini, An Jiyuan, Lorenc Michał T., Jung Hyungtaek, Sulli Maria, Aprea Giuseppe, Roden Sally, Llaca Victor, Hayashi Satomi, Asadyar Leila, LeBlanc Zacharie, Ahmed Zuba, Naim Fatima, de Campos Samanta Bolzan, Cooper Tal, de Felippes Felipe F., Dong Pengfei, Zhong Silin, Garcia-Carpintero Victor, Orzaez Diego, Dudley Kevin J., Bombarely Aureliano, Bally Julia, Winefield Christopher, Giuliano Giovanni, and Waterhouse Peter M.
RivistaNature Plants
Type of ArticleArticle

Nicotiana benthamiana is an invaluable model plant and biotechnology platform with a 3 Gb allotetraploid genome. To further improve its usefulness and versatility, we have produced high-quality chromosome-level genome assemblies, coupled with transcriptome, epigenome, microRNA and transposable element datasets, for the ubiquitously used LAB strain and a related wild accession, QLD. In addition, single nucleotide polymorphism maps have been produced for a further two laboratory strains and four wild accessions. Despite the loss of five chromosomes from the ancestral tetraploid, expansion of intergenic regions, widespread segmental allopolyploidy, advanced diploidization and evidence of recent bursts of Copia pseudovirus (Copia) mobility not seen in other Nicotiana genomes, the two subgenomes of N. benthamiana show large regions of synteny across the Solanaceae. LAB and QLD have many genetic, metabolic and phenotypic differences, including disparate RNA interference responses, but are highly interfertile and amenable to genome editing and both transient and stable transformation. The LAB/QLD combination has the potential to be as useful as the Columbia-0/Landsberg errecta partnership, utilized from the early pioneering days of Arabidopsis genomics to today. © 2023, The Author(s).


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Citation KeyRanawaka2023