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Vertical ordering sensitivity of solid supported DPPC membrane to alamethicin and the related loss of cell viability

TitoloVertical ordering sensitivity of solid supported DPPC membrane to alamethicin and the related loss of cell viability
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2015
AutoriDomenici, F., Dell'Unto F., Triggiani Doriana, Olmati C., Castellano C., Bordi F., Tiezzi A., and Congiu A.
RivistaBiochimica et Biophysica Acta - General Subjects
Volume1850
Paginazione759-768
ISSN03044165
Parole chiave1, 2-Dipalmitoylphosphatidylcholine, alamethicin, article, cell death, cell membrane, Cell Survival, cell viability, chemistry, controlled study, cytotoxicity test, dipalmitoylphosphatidylcholine, drug effects, Elasticity, Female, HeLa cell line, Hela Cells, human, human cell, Humans, hydrogen bond, Hydrophobicity, lipid bilayer, Lipid Bilayers, lipid membrane, membrane structure, phospholipid bilayer, Sensitivity analysis, surface property, thickness, X ray diffraction, X-Ray Diffraction
Abstract

Background: Experimental studies of antimicrobial peptides interacting with lipid membranes recently attracted growing interest due to their numerous biomedical applications. However, the influence of such peptides on the structural organisation of lipid membranes in connection with the actual cell response still remains an elusive issue. Methods: X-ray diffraction was employed on detecting the sensitivity of the periodical spacing of dipalmitoyl-phosphatidyl-choline stacked as solid-supported bilayers to the presence of varying amounts of the peptide alamethicin in a wide range of peptide-to-lipid molar ratios. These results were then correlated with the effects of alamethicin on biological membranes in vitro as observed by optical microscopy and microculture tetrazolium assay on the tumour cells HeLa to provide a comprehensive and quantitative analysis of these effects, based on a dose-response relationship. Results: The experiments allowed correlating the periodical spacing and the peptide-to-lipid molar ratio on alamethicin-dipalmitoyl-phosphatidyl-choline samples. Two different trends of periodical spacing vs. peptide-to-lipid molar ratio clearly appeared at low and high hydration levels, showing intriguing non-linear profiles. Unexpected correspondences were observed between the peptide-to-lipid molar ratio range where the changes in dipalmitoyl-phosphatidyl-choline structure occur and the alamethicin doses which alter the viability and the plasma membrane morphology of HeLa. Conclusions: Alamethicin might induce either mechanical or phase changes on dipalmitoyl-phosphatidyl-choline bilayers. Such easily accessible ordering information was well-calibrated to predict the alamethicin doses necessary to trigger cell death through plasma membrane alterations. General significance: This benchmark combined study may be valuable to predict bioeffects of several antimicrobial peptides of biomedical relevance. © 2015 Elsevier B.V. All rights reserved.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84921509371&doi=10.1016%2fj.bbagen.2015.01.006&partnerID=40&md5=b77e3688990df192afcfe6860b24724b
DOI10.1016/j.bbagen.2015.01.006
Citation KeyDomenici2015759