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Lactoferrin prevents invasion and inflammatory response following E. coli strain LF82 infection in experimental model of Crohn's disease

TitoloLactoferrin prevents invasion and inflammatory response following E. coli strain LF82 infection in experimental model of Crohn's disease
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2014
AutoriBertuccini, L., Costanzo Manuela, Iosi F., Tinari A., Terruzzi F., Stronati L., Aloi M., Cucchiara S., and Superti F.
RivistaDigestive and Liver Disease
Volume46
Paginazione496-504
ISSN15908658
Parole chiaveanimal, Animals, Anti-Infective Agents, antibacterial activity, antiinfective agent, antimicrobial activity, article, Bacterial Adhesion, bacterial cell, bacterial strain, bacterium adherence, blf, Bovinae, CACO 2 cell line, Caco-2 Cells, Cattle, cell adhesion, cell invasion, controlled study, Crohn disease, cytokine production, drug effects, drug efficacy, drug mechanism, electron, Electron microscopy, Escherichia coli, Escherichia coli infection, Escherichia coli Infections, fimbriae receptor, gamma interferon, Gene expression, genetics, human, human cell, Humans, immunoglobulin receptor, Immunologic, immunology, inflammation, Interferon-gamma, interleukin 1, interleukin 6, interleukin 8, Interleukin-6, Interleukin-8, Intestinal Mucosa, intestine biopsy, intestine epithelium cell, intestine mucosa, lactoferrin, mannose, Messenger, messenger RNA, metabolism, microbiology, Microscopy, minimum inhibitory concentration, physiology, priority journal, protein expression, Receptors, RNA, Scanning, Scanning electron microscopy, Transmission, Transmission electron microscopy, tumor necrosis factor alpha, Tumor Necrosis Factor-alpha, Type 1, unclassified drug
Abstract

Background: Crohn's disease is a multifactorial disease in which an aberrant immune response to commensal intestinal microbiota leads to chronic inflammation. The small intestine of patients with Crohn's disease is colonized by a group of adherent-invasive Escherichia coli strongly able to adhere and invade intestinal epithelial cells lactoferrin is an iron-binding glycoprotein known to have anti-bacterial and anti-inflammatory activities. Aims: We explore the ability of bovine lactoferrin to modulate the interactions between the adherent-invasive E. coli strain LF82 and intestinal epithelial cells as well as the inflammatory response. Methods: Bacterial adhesion and invasion assays were used to assess the antimicrobial activity of lactoferrin. Electron microscopy was used to characterize bacteria-cell interactions. The mRNA expression of pro-inflammatory cytokines was measured both in cultured cells and in biopsies taken from intestine of patients affected by Crohn's disease. Results: Lactoferrin inhibited bacterial invasion through minimally affecting adhesion. This divergence was due to a mannose-dependent lactoferrin binding to the bacterial type 1 pili and consequent bacterial aggregation on the intestinal epithelial cell surface. Expression of pro-inflammatory cytokines, such as TNF-alpha, IL-8, and IL-6, was markedly inhibited by lactoferrin both in cultured and Crohn-derived intestinal cells. Conclusions: Bovine lactoferrin might function via an antibacterial and/or anti-inflammatory mechanism in the treatment of Crohn's disease. © 2014 Editrice Gastroenterologica Italiana S.r.l.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84902543264&doi=10.1016%2fj.dld.2014.02.009&partnerID=40&md5=f560e378571fa7948638dac4d990038d
DOI10.1016/j.dld.2014.02.009
Citation KeyBertuccini2014496