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Bacterial community of industrial raw sausage packaged in modified atmosphere throughout the shelf life

TitoloBacterial community of industrial raw sausage packaged in modified atmosphere throughout the shelf life
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2018
AutoriRaimondi, Stefano, Nappi Maria Rosaria, Sirangelo Tiziana Maria, Leonardi Alan, Amaretti Alberto, Ulrici Alessandro, Magnani Rudy, Montanari Chiara, Tabanelli Giulia, Gardini Fausto, and Rossi Maddalena
RivistaInternational Journal of Food Microbiology
Volume280
Paginazione78 – 86
Type of ArticleArticle
ISSN01681605
Abstract

Ten lots of industrial raw sausages in modified atmosphere (CO2 30%, O2 70%), produced in the same plant over 7 months, were analyzed at the day after production (S samples) and at the end of shelf life (E samples), after 12 days storage at 7 °C to simulate thermal abuse. Quality of the products was generally compromised by storage at 7 °C, with only 3 E samples without alterations. During the shelf life, the pH decreased for the accumulation of acetic and lactic acids. A few biogenic amines accumulated, remaining below acceptable limits. The profile of volatile compounds got enriched with alcohols, ketones, and acids (e.g. ethanol, 2,3-butanediol, 2,3-butandione, butanoic acid) originated by bacterial metabolism. Throughout the shelf life, aerobic bacteria increased from 4.7 log to 6.6 log cfu/g, and lactic acid bacteria (LAB) from 3.7 to 8.1 log cfu/g. Staphylococci, enterobacteria, and pseudomonads passed from 3.7, 3.0, and 1.7 to 5.5, 4.8, and 3.0 log cfu/g, respectively. Dominant cultivable LAB, genotyped by RAPD-PCR, belonged to the species Lactobacillus curvatus/graminis and Lactobacillus sakei, with lower amounts of Leuconostoc carnosum and Leuconostoc mesenteroides. Brochothrix thermosphacta was the prevailing species among aerobic bacteria. The same biotypes ascribed to several different species where often found in E samples of diverse batches, suggesting a recurrent contamination from the plant of production. Profiling of 16S rRNA gene evidenced that microbiota of S samples clustered in two main groups where either Firmicutes or Bacteroidetes prevailed, albeit with taxa generally associated to the gastro-intestinal tract of mammals. The microbial diversity was lower in E samples than in S ones. Even though a common profile could not be identified, most E samples clustered together and were dominated by Firmicutes, with Lactobacillaceae and Listeriaceae as the most abundant families (mostly ascribed to Lactobacillus and Brochothrix, respectively). In a sole E sample Proteobacteria (especially Serratia) was the major phylum. © 2018 Elsevier B.V.

Note

Cited by: 27; All Open Access, Green Open Access

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85047054988&doi=10.1016%2fj.ijfoodmicro.2018.04.041&partnerID=40&md5=5dee79d830d92acc9510b215b0c38124
DOI10.1016/j.ijfoodmicro.2018.04.041
Citation KeyRaimondi201878
PubMed ID29783046