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Color mutations alter the biochemical composition in the san marzano tomato fruit

TitleColor mutations alter the biochemical composition in the san marzano tomato fruit
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2020
AuthorsDono, G., Rambla J.L., Frusciante Sarah, Granell A., Diretto Gianfranco, and Mazzucato A.
KeywordsAmino Acid, article, biochemical composition, bioinformatics, Chlorophyll, down regulation, fatty acid derivative, formononetin, fruit, gene mutation, genotype, ionization, kaempferol, Mass Spectrometry, metabolomics, nonhuman, nutritional value, phenylalanine derivative, pigmentation, plant epidermis, plant growth, Principal component analysis, quinone derivative, Retention time, Tomato, upregulation, xanthophyll

San Marzano (SM) is a traditional Italian landrace characterized by red elongated fruits, originating in the province of Naples (Italy) and cultivated worldwide. Three mutations, yellow flesh (r), green flesh (gf ) and colorless fruit epidermis (y) were introduced into SM by backcross and the resulting introgression lines (ILs) produced the expected yellow, brown and pink fruit variants. In addition, ILs carrying double combinations of those mutations were obtained. The six ILs plus the SM reference were analyzed for volatile (VOC), non-polar (NP) and polar (P) metabolites. Sixty-eight VOCs were identified, and several differences evidenced in the ILs; overall gf showed epistasis over r and y and r over y. Analysis of the NP component identified 54 metabolites; variation in early carotenoids (up to lycopene) and chlorophylls characterized respectively the ILs containing r and gf. In addition, compounds belonging to the quinone and xanthophyll classes were present in genotypes carrying the r mutation at levels higher than SM. Finally, the analysis of 129 P metabolites evidenced different levels of vitamins, amino acids, lipids and phenylpropanoids in the ILs. A correlation network approach was used to investigate metabolite–metabolite relationships in the mutant lines. Altogether these differences potentially modified the hedonistic and nutritional value of the berry. In summary, single and combined mutations in gf, r and y generated interesting visual and compositional diversity in the SM landrace, while maintaining its original typology. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.


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Citation KeyDono2020