Sorry, you need to enable JavaScript to visit this website.

Xenobiotic activity in serum and sperm chromatin integrity in European and inuit populations.

TitleXenobiotic activity in serum and sperm chromatin integrity in European and inuit populations.
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2008
AuthorsKrüger, Tanja, Spanò M., Long Manhai, Eleuteri Patrizia, Rescia Michele, Hjelmborg Philip S., Manicardi Gian-Carlo, Bizzaro Davide, Giwercman Alexander, Toft Gunnar, Bonde Jens Peter, and Bonefeld-Jorgensen Eva C.
JournalMol Reprod Dev
Date Published2008 Apr
Keywordschromatin, Cohort studies, DNA fragmentation, environmental exposure, Environmental Pollutants, European Continental Ancestry Group, Humans, Inuits, male, Receptors, Androgen, Receptors, Aryl Hydrocarbon, Receptors, Estrogen, Reproducibility of Results, Spermatozoa, Xenobiotics

Lipophilic persistent organic pollutants (POPs) are ubiquitous in the environment and suspected to interfere with hormone activities and reproduction. In previous studies we demonstrated that POP exposure can affect sperm DNA integrity and differences between Inuits and Europeans in sperm DNA integrity and xenobiotic activity were observed. The aim of this study was to investigate possible relations between human sperm chromatin integrity and the xenobiotic serum activity of lipophilic POPs assessed as effects on the estrogen (ER), androgen (AR), and/or aryl hydrocarbon (AhR) receptors. Human sperm chromatin integrity was assessed as DNA fragmentation index (%DFI) and high DNA stainability (%HDS) using the flow cytometric sperm chromatin structure assay (SCSA). Xenobiotic receptor activities were determined using chemically activated luciferase gene expression (CALUX) assay. The study included 53 Greenlandic Inuits and 247 Europeans (Sweden, Warsaw (Poland) and Kharkiv (Ukraine)). A heterogeneous pattern of correlations was found. For Inuits, ER and AhR activities and %DFI were inversely correlated, whereas a positive correlation between AR activity and %DFI was found for Europeans. In contrast, no correlation between receptor activities and %HDS was observed for Inuits but for Europeans positive and negative correlations were observed between ER and AR activities and %HDS, respectively. We suggest that the different patterns of xenobiotic serum activities, in combination with diet associated factors and/or genetics, might be connected to the observed differences in sperm chromatin integrity between the Inuits and Europeans.


cited By 14

Alternate JournalMol. Reprod. Dev.
Citation Key4914
PubMed ID18076054