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Direct and delayed X-ray-induced DNA damage in male mouse germ cells.

TitleDirect and delayed X-ray-induced DNA damage in male mouse germ cells.
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2012
AuthorsCordelli, Eugenia, Eleuteri Patrizia, Grollino Maria Giuseppa, Benassi Barbara, Blandino Giovanni, Bartoleschi Cecilia, Pardini Maria Chiara, Di Caprio Edoardo Vittorio, Spanò M., Pacchierotti Francesca, and Villani Paola
JournalEnviron Mol Mutagen
Date Published2012 Jul
KeywordsAnimals, DNA damage, DNA repair, male, Mice, Mice, Inbred C57BL, Mutagenicity Tests, mutation, spermatogenesis, Spermatozoa, testis, X-Rays

Sperm DNA integrity is essential for the accurate transmission of paternal genetic information. Various stages of spermatogenesis are characterized by large differences in radiosensitivity. Differentiating spermatogonia are susceptible to radiation-induced cell killing, but some of them can repair DNA damage and progress through differentiation. In this study, we applied the neutral comet assay, immunodetection of phosphorylated H2AX (γ-H2AX) and the Sperm Chromatin Structure Assay (SCSA) to detect DNA strand breaks in testicular cells and spermatozoa at different times following in vivo X-ray irradiation. Radiation produced DNA strand breaks in testicular cells that were repaired within the first few hours after exposure. Spermatozoa were resistant to the induction of DNA damage, but non-targeted DNA lesions were detected in spermatozoa derived from surviving irradiated spermatogonia. These lesions formed while round spermatids started to elongate within the testicular seminiferous tubules. The transcription of pro-apoptotic genes at this time was also enhanced, suggesting that an apoptotic-like process was involved in DNA break production. Our results suggest that proliferating spermatogonia retain a memory of the radiation insult that is recognized at a later developmental stage and activates a process leading to DNA fragmentation.


cited By 10

Alternate JournalEnviron. Mol. Mutagen.
Citation Key4908
PubMed ID22730201