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Flow cytometric and histological assessment of 1,2:3,4-diepoxybutane toxicity on mouse spermatogenesis

TitleFlow cytometric and histological assessment of 1,2:3,4-diepoxybutane toxicity on mouse spermatogenesis
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication1996
AuthorsSpanò, M., Bartoleschi Cecilia, Cordelli Eugenia, Leter Giorgio, Segre L., Mantovani A., Fazzi P., and Pacchierotti Francesca
JournalJournal of Toxicology and Environmental Health
Keywordsanimal cell, animal experiment, animal model, Animals, article, butadiene diepoxide, chromatin, controlled study, Cytotoxicity, dose response, Dose-Response Relationship, Drug, Epoxy Compounds, Flow cytometry, genotoxicity, histology, histopathology, male, Mice, mouse, Mutagens, nonhuman, priority journal, Reproducibility of Results, spermatid, Spermatids, Spermatocytes, spermatogenesis, Spermatogonia, spermatogonium, Spermatozoa, testis

The effects of diepoxybutane (DEB) on mouse reproductive cells have been investigated by flow cytometric and histological description of testicular cell populations and alterations of sperm chromatin packaging. Mice were treated with single intraperitoneal injections of DEB, with doses ranging between 8.5 and 78 mg/kg (100-900 μM), and were killed after 7, 14, 21, 28, or 35 d. Dose-dependent reductions of tetraploid cells, round spermatids, and elongated spermatids were detected at 7, 21, and 28 d, respectively, reflecting cytotoxic damage on the differentiating spermatogonia compartment. The dose necessary to reduce the number of differentiating spermatogonia to half the control value was estimated equal to 650 μM or 55 mg/kg. Stem cells were not affected by this treatment. Histological section of seminiferous tubules showed depletion of spermatids and reduction of the secondary spermatocyte layers. In addition, a high although not statistically significant frequency of sperm with altered chromatin packaging was detected after DEB treatment. DEB is one of the key metabolites of butadiene, which is a compound of high environmental and occupational concern. These results contribute to the assessment of the reproductive health impact of butadiene in humans.


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Citation KeySpano1996423