| Title | Biodistribution and acute toxicity of a nanofluid containing manganese iron oxide nanoparticles produced by a mechanochemical process |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2014 |
| Authors | Bellusci, M., La Barbera A., Padella F., Mancuso Mariateresa, Pasquo A., Grollino Maria Giuseppa, Leter Giorgio, Nardi Elisa, Cremisini C., Giardullo Paola, and Pacchierotti Francesca |
| Journal | International Journal of Nanomedicine |
| Volume | 9 |
| Pagination | 1919-1929 |
| ISSN | 11769114 |
| Keywords | 3 (4, 3T3 cell line, 5 dimethyl 2 thiazolyl) 2, 5 diphenyltetrazolium bromide, acute toxicity, animal, animal cell, animal tissue, Animals, antibody specificity, article, BALB 3T3 Cells, bioaccumulation, Biosafety, Brain, Cell Survival, chemistry, colloid, Colloids, concentration response, Contrast Media, contrast medium, controlled study, Cytotoxicity, diffusion, dose response, Dose-Response Relationship, Drug, Drug Compounding, drug effects, drug formulation, Female, fibroblast, Flow cytometry, histopathology, in vitro study, in vivo study, kidney, LD50, Lethal Dose 50, light scattering, Liver, magnetite nanoparticle, Magnetite Nanoparticles, Manganese, manganese iron oxide nanoparticle, Mass Spectrometry, Materials testing, Mechanical, mechanical stress, mechanotransduction, Mice, mouse, Nanobiotechnology, nanoparticle, nonhuman, Organ Specificity, procedures, solution and solubility, Solutions, Spleen, stress, survival rate, synthesis, Tissue Distribution, Toxicity, unclassified drug |
| Abstract | Superparamagnetic iron oxide nanoparticles are candidate contrast agents for magnetic resonance imaging and targeted drug delivery. Biodistribution and toxicity assessment are critical for the development of nanoparticle-based drugs, because of nanoparticle-enhanced biological reactivity. Here, we investigated the uptake, in vivo biodistribution, and in vitro and in vivo potential toxicity of manganese ferrite (MnFe2O4) nanoparticles, synthesized by an original high-yield, low-cost mechanochemical process. Cultures of murine Balb/3T3 fibroblasts were exposed for 24, 48, or 72 hours to increasing ferrofluid concentrations. Nanoparticle cellular uptake was assessed by flow-cytometry scatter-light measurements and microscopy imaging after Prussian blue staining; cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and colony-forming assays. After a single intravenous injection, in vivo nanoparticle biodistribution and clearance were evaluated in mice by Mn spectrophotometric determination and Prussian blue staining in the liver, kidneys, spleen, and brain at different posttreatment times up to 21 days. The same organs were analyzed for any possible histopathological change. The in vitro study demonstrated dose-dependent nanoparticle uptake and statistically significant cytotoxic effects from a concentration of 50 μg/mL for the MTT assay and 20 μg/mL for the colony-forming assay. Significant increases in Mn concentrations were detected in all analyzed organs, peaking at 6 hours after injection and then gradually declining. Clearance appeared complete at 7 days in the kidneys, spleen, and brain, whereas in the liver Mn levels remained statistically higher than in vehicle-treated mice up to 3 weeks postinjection. No evidence of irreversible histopathological damage to any of the tested organs was observed. A comparison of the lowest in vitro toxic concentration with the intravenously injected dose and the administered dose of other ferrofluid drugs currently in clinical practice suggests that there might be sufficient safety margins for further development of our formulation. © 2014 Bellusci et al. |
| Notes | cited By 15 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84899154134&doi=10.2147%2fIJN.S56394&partnerID=40&md5=c62445d2dead562f15cf9939f53e0882 |
| DOI | 10.2147/IJN.S56394 |
| Citation Key | Bellusci20141919 |
