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Towards Complex Tissues Replication: Multilayer Scaffold Integrating Biomimetic Nanohydroxyapatite/Chitosan Composites

TitoloTowards Complex Tissues Replication: Multilayer Scaffold Integrating Biomimetic Nanohydroxyapatite/Chitosan Composites
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2024
AutoriPalazzo, Barbara, Scialla S., Barca A., Sercia L., Izzo D., Gervaso F., and Scalera F.
RivistaBioengineering
Volume11
ISSN23065354
Abstract

This study explores an approach to design and prepare a multilayer scaffold mimicking interstratified natural tissue. This multilayer construct, composed of chitosan matrices with graded nanohydroxyapatite concentrations, was achieved through an in situ biomineralization process applied to individual layers. Three distinct precursor concentrations were considered, resulting in 10, 20, and 30 wt% nanohydroxyapatite content in each layer. The resulting chitosan/nanohydroxyapatite (Cs/n-HAp) scaffolds, created via freeze-drying, exhibited nanohydroxyapatite nucleation, homogeneous distribution, improved mechanical properties, and good cytocompatibility. The cytocompatibility analysis revealed that the Cs/n-HAp layers presented cell proliferation similar to the control in pure Cs for the samples with 10% n-HAp, indicating good cytocompatibility at this concentration, while no induction of apoptotic death pathways was demonstrated up to a 20 wt% n-Hap concentration. Successful multilayer assembly of Cs and Cs/n-HAp layers highlighted that the proposed approach represents a promising strategy for mimicking multifaceted tissues, such as osteochondral ones. © 2024 by the authors.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85194086233&doi=10.3390%2fbioengineering11050471&partnerID=40&md5=5780397b6b00dd075b8d1d2f0f4c863d
DOI10.3390/bioengineering11050471
Citation KeyPalazzo2024