Sorry, you need to enable JavaScript to visit this website.

CD4+CD25lowGITR+ cells: A novel human CD4+ T-cell population with regulatory activity

TitoloCD4+CD25lowGITR+ cells: A novel human CD4+ T-cell population with regulatory activity
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2011
AutoriBianchini, Rodolfo, Bistoni Onelia, Alunno Alessia, Petrillo Maria Grazia, Ronchetti Simona, Sportoletti Paolo, Bocci Elena Bartoloni, Nocentini Giuseppe, Gerli Roberto, and Riccardi Carlo
RivistaEuropean Journal of Immunology
Volume41
Paginazione2269 – 2278
Type of ArticleArticle
ISSN15214141
Parole chiaveadult, animal cell, Animals, Antigens, article, blood, CD, CD4+ CD25+ T lymphocyte, CD4+ T lymphocyte, CD4-Positive T-Lymphocytes, cell function, cell proliferation, controlled study, cytotoxic T lymphocyte antigen 4, Flow cytometry, Gene expression, glucocorticoid induced tumor necrosis factor receptor, Glucocorticoid-Induced TNFR-Related Protein, human, human cell, Humans, Immunologic Memory, Immunomodulation, Immunophenotyping, immunoregulation, interleukin 10, interleukin 2 receptor alpha, interleukin 7 receptor, Interleukin-10, Interleukin-2 Receptor alpha Subunit, lymphocyte proliferation, memory T lymphocyte, Mice, Middle Aged, nonhuman, priority journal, protein expression, protein function, Regulatory, regulatory T lymphocyte, Reverse Transcriptase Polymerase Chain Reaction, T lymphocyte activation, T lymphocyte subpopulation, T-Lymphocyte Subsets, T-Lymphocytes, transcription factor FOXP3, transforming growth factor beta, young adult
Abstract

Treg subsets play a role in sustaining peripheral tolerance, are characterized by markers such as forkhead winged-helix transcription factor (FOXP3) and CD25, and produce suppressive cytokines, such as IL-10 and TGF-β. Glucocorticoid-induced TNF receptor family-related (GITR) protein has been suggested to regulate Treg activity in mice. The aim of our study was to investigate GITR expression in human CD4+ T lymphocytes and its possible role in Treg function. Results indicate that a subset of CD4+ T cells in the peripheral blood expresses GITR and low levels of CD25 (CD4+CD25lowGITR+). These cells show Treg features as they express FOXP3, IL-10, TGF-β and are anergic but, as opposed to natural Tregs, express low levels of CTLA-4 and are CD127high. CD4+CD25lowGITR+ cells represent a low percentage of the CD4+ T-cell population (0.32-1.74%) and are mostly memory cells. Functional experiments demonstrated that CD4+CD25lowGITR+ cells have relevant suppressive activity that depends on TGF-β. Moreover, an anti-GITR Ab inhibited their suppressive activity, as observed in CD4+CD25+ murine Tregs. Taken together, these data indicate that human CD4+CD25lowGITR+ cells represent a distinct Treg subpopulation. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Note

Cited by: 53; All Open Access, Bronze Open Access

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-79960718028&doi=10.1002%2feji.201040943&partnerID=40&md5=63b6f207b19f780e077221ca7ae91264
DOI10.1002/eji.201040943
Citation KeyBianchini20112269
PubMed ID21557210