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The hypoxic expression of the glucose transporter RAG1 reveals the role of the bHLH transcription factor Sck1 as a novel hypoxic modulator in Kluyveromyces lactis

TitoloThe hypoxic expression of the glucose transporter RAG1 reveals the role of the bHLH transcription factor Sck1 as a novel hypoxic modulator in Kluyveromyces lactis
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2019
AutoriSantomartino, Rosa, Ottaviano Daniela, Camponeschi Ilaria, Landicho Tracy Ann Alcarp, Falato Luca, Visca Andrea, Soulard Alexandre, Lemaire Marc, and Bianchi Michele Maria
RivistaFEMS Yeast Research
Volume19
Paginazionefoz041
ISSN15671356
Parole chiaveanaerobic growth, Anaerobiosis, article, beta galactosidase, controlled study, Facilitative, Fungal, fungal protein, Fungal Proteins, fungal strain, gene expression regulation, Genetic engineering, genetic transcription, genetics, Glucose, glucose transport, Glucose Transport Proteins, glucose transporter, glycolysis, Kluyveromyces, Kluyveromyces lactis, metabolism, mutation, nonhuman, Oxygen, promoter region, protein expression, protein function, quantitative analysis, RAG1 protein, signal transduction, transcription factor, transcription factor sck1, Transcription Factors, unclassified drug
Abstract

Glucose is the preferred nutrient for most living cells and is also a signaling molecule that modulates several cellular processes. Glucose regulates the expression of glucose permease genes in yeasts through signaling pathways dependent on plasma membrane glucose sensors. In the yeast Kluyveromyces lactis, sufficient levels of glucose induction of the low-affinity glucose transporter RAG1 gene also depends on a functional glycolysis, suggesting additional intracellular signaling. We have found that the expression of RAG1 gene is also induced by hypoxia in the presence of glucose, indicating that glucose and oxygen signaling pathways are interconnected. In this study we investigated the molecular mechanisms underlying this crosstalk. By analyzing RAG1 expression in various K. lactis mutants, we found that the bHLH transcriptional activator Sck1 is required for the hypoxic induction of RAG1 gene. The RAG1 promoter region essential for its hypoxic induction was identified by promoter deletion experiments. Taken together, these results show that the RAG1 glucose permease gene is synergistically induced by hypoxia and glucose and highlighted a novel role for the transcriptional activator Sck1 as a key mediator in this mechanism. © 2019 FEMS.

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cited By 2

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85069236476&doi=10.1093%2ffemsyr%2ffoz041&partnerID=40&md5=16e9fa84d9623ab3f6279893c8b233a9
DOI10.1093/femsyr/foz041
Citation Keysantomartino2019hypoxic