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Bioaugmentation with a consortium of bacterial sodium lauryl ether sulfate-degraders for remediation of contaminated soils

TitoloBioaugmentation with a consortium of bacterial sodium lauryl ether sulfate-degraders for remediation of contaminated soils
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2021
AutoriRolando, Ludovica, Caracciolo Anna Barra, Grenni Paola, Mariani Livia, Rauseo Jasmin, Spataro Francesca, Garbini Gian Luigi, Visca Andrea, and Patrolecco Luisa
RivistaFrontiers in Microbiology
Volume12
Paginazione740118
Data di pubblicazioneOct-09-2022
ISSN1664302X
Parole chiaveacute toxicity, Aliivibrio fischeri, article, bacterial growth, bacterium culture, Bioaugmentation, Biodegradation, Bioluminescence, Bioremediation, cell growth, cell viability, colony forming unit, controlled study, Cytotoxicity, dodecyl sulfate sodium, EC20, EC50, ecotoxicology, epifluorescence microscopy, foaming, foaming agent, growth rate, high performance liquid chromatography, Inoculation, limit of detection, liquid liquid extraction, Microbial community, microbial consortium, nonhuman, optical density, population abundance, toxicity testing
Abstract

The anionic surfactant sodium lauryl ether sulfate (SLES) is the main component of most commercial foaming agents (FAs) used in the excavation of highway and railway tunnels with Earth pressure balance-tunnel boring machines (EPB-TBMs). Several hundreds of millions of tons of spoil material, consisting of soil mixed with FAs, are produced worldwide, raising the issue of their handling and safe disposal. Reducing waste production and reusing by-products are the primary objectives of the “circular economy,” and in this context, the biodegradation of SLES becomes a key question in reclaiming excavated soils, especially at construction sites where SLES degradation on the spot is not possible because of lack of space for temporary spoil material storage. The aim of the present work was to apply a bacterial consortium (BC) of SLES degraders to spoil material excavated with an EPB-TBM and coming from a real construction site. For this purpose, the BC capability to accelerate SLES degradation was tested. Preliminary BC growth, degradation tests, and ecotoxicological evaluations were performed on a selected FA. Subsequently, a bioaugmentation experiment was conducted; and the microbial abundance, viability, and SLES concentrations in spoil material were evaluated over the experimental time (0.5, 3, 6, 24, 48, and 144 h). Moreover, the corresponding aqueous elutriates were extracted from all the soil samples and analyzed for SLES concentration and ecotoxicological evaluations with the bacterium Aliivibrio fischeri. The preliminary experiments showed the BC capability to grow under 14 different concentrations of the FA. The maximum BC growth rates and degradation efficiency (100%) were achieved with initial SLES concentrations of 125, 250, and 500 mg/L. The subsequent bioaugmentation of the spoil material with BC significantly (sixfold) improved the degradation time of SLES (DT50 1 day) compared with natural attenuation (DT50 6 days). In line with this result, neither SLES residues nor toxicity was recorded in the soil extracts showing the spoil material as a by-product promptly usable. The bioaugmentation with BC can be a very useful for cleaning spoil material produced in underground construction where its temporary storage (for SLES natural biodegradation) is not possible. © Copyright © 2021 Rolando, Barra Caracciolo, Grenni, Mariani, Rauseo, Spataro, Garbini, Visca and Patrolecco.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85116870578&doi=10.3389%2ffmicb.2021.740118&partnerID=40&md5=31af5eba4f08a055cb35dbaec0d5e824
DOI10.3389/fmicb.2021.740118
Titolo breveFront. Microbiol.
Citation Keyrolando2021bioaugmentation