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Proteomic analysis of the plant-virus interaction in cucumber mosaic virus (CMV) resistant transgenic tomato

TitoloProteomic analysis of the plant-virus interaction in cucumber mosaic virus (CMV) resistant transgenic tomato
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2010
AutoriDi Carli, M., Villani Maria Elena, Bianco L., Lombardi R., Perrotta Gaetano, Benvenuto Eugenio, and Donini Marcello
RivistaJournal of Proteome Research
Volume9
Paginazione5684-5697
ISSN15353893
Parole chiavearticle, coat protein, comparative study, controlled study, cucumber mosaic virus, Cucumovirus, down regulation, electrospray mass spectrometry, Genetic engineering, Genetically Modified, Host-Pathogen Interactions, Immunity, Immunoglobulin Fragments, liquid chromatography, Lycopersicon esculentum, nonhuman, photosynthesis, plant defense, Plant Diseases, plant leaf, Plant leaves, plant metabolism, Plant Proteins, Plant Viruses, Plants, priority journal, protein analysis, protein expression, protein function, proteome, Proteomics, Tandem Mass Spectrometry, Tomato, transgenic plant, two dimensional gel electrophoresis, virus cell interaction, virus infection, virus resistance, wild type
Abstract

Cucumber mosaic virus (CMV), a member of the Cucumovirus genus, is the causal agent of several plant diseases in a wide range of host species, causing important economic losses in agriculture. Because of the lack of natural resistance genes in most crops, different genetic engineering strategies have been adopted to obtain virus-resistant plants. In a previous study, we described the engineering of transgenic tomato plants expressing a single-chain variable fragment antibody (scFv G4) that are specifically protected from CMV infection. In this work, we characterized the leaf proteome expressed during compatible plant-virus interaction in wild type and transgenic tomato. Protein changes in both inoculated and apical leaves were revealed using two-dimensional gel electrophoresis (2-DE) coupled to differential in gel electrophoresis (DIGE) technology. A total of 2084 spots were detected, and 50 differentially expressed proteins were identified by nanoscale liquid chromatographic-electrospray ionization-ion trap-tandem mass spectrometry (nLC-ESI-IT-MS/MS). The majority of these proteins were related to photosynthesis (38%), primary metabolism (18%), and defense activity (14%) and demonstrated to be actively down regulated by CMV in infected leaves. Moreover, our analysis revealed that asymptomatic apical leaves of transgenic inoculated plants had no protein profile alteration as compared to control wild type uninfected plants demonstrating that virus infection is confined to the inoculated leaves and systemic spread is hindered by the CMV coat protein (CP)-specific scFv G4 molecules. Our work is the first comparative study on compatible plant-virus interactions between engineered immunoprotected and susceptible wild type tomato plants, contributing to the understanding of antibody-mediated disease resistance mechanisms. © 2010 American Chemical Society.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-78149377372&doi=10.1021%2fpr100487x&partnerID=40&md5=b63354220c06cf32c391961df727d06e
DOI10.1021/pr100487x
Citation KeyDiCarli20105684