Pyrogenic synthetic amorphous silica (NM-203): Genotoxicity in rats following sub-chronic oral exposure

TitlePyrogenic synthetic amorphous silica (NM-203): Genotoxicity in rats following sub-chronic oral exposure
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2022
AuthorsVillani, Paola, Eleuteri Patrizia, Pacchierotti Francesca, Maranghi F., Tassinari R., Narciso L., Tait S., Lori G., Andreoli C., Huet S., Jarry G., Fessard V., and Cordelli E.
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume876-877
ISSN13835718
Abstract

The genotoxicity of nano-structured synthetic amorphous silica (SAS), a common food additive, was investigated in vivo in rats. A 90-day oral toxicity study was performed according to OECD test guideline 408 and the genotoxicity of pyrogenic SAS nanomaterial NM-203 was assessed in several organs, using complementary tests. Adult Sprague-Dawley rats of both sexes were treated orally for 90 days with 0, 2, 5, 10, 20, or 50 mg SAS/kg bw per day. Dose levels were selected to approximate expected human dietary exposures to SAS. DNA strand breaks were evaluated by the comet assay in blood, bone marrow, liver, and spleen according to OECD test guideline 489; mutations induced in bone marrow precursors of erythrocytes were assessed by the Pig-a assay and chromosome/ genome damage by the micronucleus assay in blood (OECD test guideline 474) and colon. No treatment-related increases of gene (Pig-a) or chromosome/genome (micronucleus) mutations were detected in the blood. The percentage of micronucleated cells was not increased in the colon of treated rats. Among the organs analyzed by the comet assay, the spleen was the only target showing a weak but biologically relevant genotoxic effect. © 2022 Elsevier B.V.

Notes

cited By 0

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85123858963&doi=10.1016%2fj.mrgentox.2022.503458&partnerID=40&md5=4e1af72b13740169139aa8ee6457cf1a
DOI10.1016/j.mrgentox.2022.503458