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Early embryo exposure to assisted reproductive manipulation induced subtle changes in liver epigenetics with no apparent negative health consequences in rabbit

TitleEarly embryo exposure to assisted reproductive manipulation induced subtle changes in liver epigenetics with no apparent negative health consequences in rabbit
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2021
AuthorsGarcia-Dominguez, X., Diretto Gianfranco, Peñaranda D.S., Frusciante Sarah, Garcia-Carpintero V., Canizares J., Vicente J.S., and Marco-Jiménez F.
JournalInternational Journal of Molecular Sciences
Volume22
Issue18
Pagination9716
Date PublishedJan-09-2021
ISSN16616596
Keywordsanimal, Animals, Assisted, biosynthesis, Breeding, DNA Methylation, Embryo, Embryo Transfer, embryology, Epigenesis, epigenetics, Epigenomics, Female, Genetic, genetic epigenesis, Genome, human, Humans, infertility therapy, Leporidae, lipid metabolism, Liver, male, Mammalian, mammalian embryo, metabolism, metabolome, pregnancy, procedures, proteome, Proteomics, Rabbits, Reproduction, Reproductive Techniques, steroid, Steroids, vitrification
Abstract

Embryo manipulation is a requisite step in assisted reproductive technology (ART). There-fore, it is of great necessity to appraise the safety of ART and investigate the long-term effect, including lipid metabolism, on ART-conceived offspring. Augmenting our ART rabbit model to investigate lipid metabolic outcomes in offspring longitudinally, we detected variations in hepatic DNA methy-lation ART offspring in the F3 generation for embryonic exposure (multiple ovulation, vitrification and embryo transfer). Through adult liver metabolomics and proteomics, we identified changes mainly related to lipid metabolism (e.g., polyunsaturated fatty acids, steroids, steroid hormone). We also found that DNA methylation analysis was linked to changes in lipid metabolism and apoptosis genes. Nevertheless, these differences did not apparently alter the general health status. Thus, our findings suggest that ART is likely to be a player in embryo epigenetic events related to hepatic homeostasis alteration in adulthood. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85114343428&doi=10.3390%2fijms22189716&partnerID=40&md5=79072497296b3dc5647fec56d74a3873
DOI10.3390/ijms22189716
Short TitleIJMS
Citation KeyGarcía-Domínguez2021