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Leaf proteome analysis of transgenic plants expressing antiviral antibodies

TitleLeaf proteome analysis of transgenic plants expressing antiviral antibodies
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2009
AuthorsCarli, M.D., Villani Maria Elena, Renzone G., Nardi Luca, Pasquo A., Franconi Rosella, Scaloni A., Benvenuto Eugenio, and Desiderio Angiola
JournalJournal of Proteome Research
KeywordsAgrobacterium tumefaciens, Antibodies, article, Biosafety, controlled study, cucumber mosaic virus, defense mechanism, electrospray mass spectrometry, Genetically Modified, ion trap mass spectrometry, liquid chromatography, Lycopersicon esculentum, matrix assisted laser desorption ionization time of flight mass spectrometry, Molecular Sequence Data, Nicotiana benthamiana, nonhuman, photosynthesis, plant disease, plant leaf, Plant leaves, Plant Proteins, Plants, pleiotropy, Principal component analysis, priority journal, protein analysis, protein expression, protein variant, proteome, Proteomics, quantitative analysis, Rhizobium radiobacter, Tobacco, Tomato, Tomato spotted wilt virus, transgenic plant, two dimensional gel electrophoresis, Viral, virus antibody, virus infection

The expression of exogenous antibodies in plant is an effective strategy to confer protection against viral infection or to produce molecules with pharmaceutical interest. However, the acceptance of the transgenic technology to obtain self-protecting plants depends on the assessment of their substantial equivalence compared to non-modified crops with an established history of safe use. In fact, the possibility exists that the introduction of transgenes in plants may alter expression of endogenous genes and/or normal production of metabolites. In this study, we investigated whether the expression in plant of recombinant antibodies directed against viral proteins may influence the host leaf proteome. Two transgenic plant models, generated by Agrobacterium tumefaciens-medlated transformation, were analyzed for this purpose, namely, Lycopersicon esculentum cv. MicroTom and Nicotiana benthamiana, expressing recombinant antibodies against cucumber mosaic virus and tomato spotted wilt virus, respectively. To obtain a significant representation of plant proteomes, optimized extraction procedures have been devised for each plant species. The proteome repertoire of antibody-expressing and control plants was compared by 2-DE associated to DIGE technology. Among the 2000 spots detected within the gels, about 10 resulted differentially expressed in each transgenic model and were identified by MALDI-TOF PMF and μLC-ESI-IT-MS/MS procedures. Protein variations were restricted to a limited number of defined differences with an average ratio below 2.4. Most of the differentially expressed proteins were related to photosynthesis or defense function. The overall results suggest that the expression of recombinant antibodies in both systems does not significantly alter the leaf proteomic profile, contributing to assess the biosafety of resistant plants expressing antiviral antibodies. © 2009 American Chemical Society.


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Citation KeyCarli2009838