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Impact of zinc oxide nanoparticles on an in vitro model of the human air-blood barrier

TitleImpact of zinc oxide nanoparticles on an in vitro model of the human air-blood barrier
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2017
AuthorsBengalli, R., Gualtieri Maurizio, Capasso L., Urani C., and Camatini M.
JournalToxicology Letters
Volume279
Pagination22-32
ISSN03784274
Keywordsair blood barrier, article, autacoid, Blood-Air Barrier, capillary endothelial cell, Cell Line, cell viability, Cells, coculture, Coculture Techniques, comparative study, confocal microscopy, controlled study, cytokine release, dose response, Dose-Response Relationship, Drug, drug effects, Electric conductivity, Electric resistance, Endothelial Cells, endothelium cell, enzyme linked immunosorbent assay, epithelial cell line, Epithelial Cells, epithelium cell, human, human cell, Humans, hydrodynamics, ICAM1 protein, IL6 protein, IL8 protein, in vitro study, Inflammation Mediators, intercellular adhesion molecule 1, Intercellular Adhesion Molecule-1, interleukin 6, interleukin 8, Interleukin-6, Interleukin-8, lung gas exchange, lung toxicity, metabolism, metal nanoparticle, Metal nanoparticles, metallothionein, monoculture, monocyte, Monocytes, particle size, Pathology, permeability, permeability barrier, priority journal, protein expression, protein ZO1, soluble intercellular adhesion molecule 1, soluble vascular cell adhesion molecule 1, supernatant, suspension, tight junction, Tight Junctions, TJP1 protein, Transmission electron microscopy, Tumor, tumor cell line, unclassified drug, upregulation, vascular cell adhesion molecule 1, Vascular Cell Adhesion Molecule-1, Western blotting, Zinc oxide, zinc oxide nanoparticle, Zonula Occludens-1 Protein
Abstract

The inhalation of zinc oxide nanoparticles (nZnO) may induce systemic diseases, damages to the alveolar epithelium and inflammatory response to endothelial cells. In this work the use of an in vitro air-blood barrier (ABB) model provided a tool to elucidate the biological mechanisms underlying the potential effects of inhaled nanoparticles (NPs). The ABB model used is composed of a Transwell co-culture of a lung epithelial cell line (NCI-H441) and an immortalized pulmonary microvascular endothelial cell line (HPMEC-ST1.6R). In addition, a tri-culture model was developed by adding monocytes (THP-1) on the basal compartment of the inserts. These models have been set up to analyse the importance of the interplay among the different cell types on various responses after nZnO exposure: inflammation, endothelial damage and modulation of the immune system. The barrier integrity was assessed by measuring the transepithelial electrical resistance (TEER); the pro-inflammatory and immune cells responses were analysed by ELISA. The results have evidenced that nZnO do not affect the barrier integrity, since no TEER reduction was measured after 24 h of exposure, but an activation of endothelial cells, which released pro-inflammatory mediators (IL-6, IL-8), and endothelial dysfunction markers (sICAM-1 and sVCAM-1) were induced. These results confirm that apical exposure to NPs promote endothelium activation. The in vitro-ABB model here used is thus a useful tool able to evidence the interaction between lung epithelium and endothelium in inducing biological response, and the role of endothelium dysfunction following NPs inhalation. © 2017 Elsevier B.V.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85025684822&doi=10.1016%2fj.toxlet.2017.07.877&partnerID=40&md5=0c026d3eb16e0f75b2f1480dce14e73e
DOI10.1016/j.toxlet.2017.07.877
Citation KeyBengalli201722