Sorry, you need to enable JavaScript to visit this website.

Transgenerational effects of BPA on female reproduction

TitleTransgenerational effects of BPA on female reproduction
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2019
AuthorsSantangeli, S., Consales Claudia, Pacchierotti Francesca, Habibi H.R., and Carnevali O.
JournalScience of the Total Environment
ISSN00489697
KeywordsAlkylation, Bis-phenol a, DNA Methylation, Female reproduction, Gene expression, Histone modification, Methylation, Phenols, transgenerational inheritance
Abstract

Bisphenol A (BPA) is an abundant environmental contaminant and studies have shown the presence of BPA in the urine of over 90% of population tested in Canada and USA. In addition to its reported harmful effects, there is concern for its transgenerational effects. For a compound to induce transgenerational effect, an epigenetic mark should be mitotically and meiotically stable without reprogramming in primordial germ cells and post fertilization embryos. In the present study, female zebrafish were treated with an environmental dose (20 μg/L) of BPA and then crossed with untreated males. To assess epigenetic effects, transcript levels of several genes involved in female reproduction were measured in adult and in 24 hpf embryos up to F3 generation. Exposure to BPA affected adult female fertility up to F2 generation. In F0, F1 and F2 ovaries transcript levels for several genes involved in reproduction, including esr, star, lhcgr and fshr were affected. To investigate epigenetic mechanisms of gene expression modulation, we studied promoter DNA methylation. Among genes involved in gonadal differentiation, amh transcript level was reduced in 24 hpf embryos, up to the F3 generation. Variation in amh transcript level was associated with hyper-methylation of its promoter and changes in H3K4me3/H3K27me3 enrichment, coherent with gene silencing. The findings provide evidence for transgenerational effects of BPA in zebrafish and demonstrate that amh is susceptible to stable epigenetic alterations. Capsule: Transgenerational effects of BPA on female reproductive physiology. © 2019 Elsevier B.V.

Notes

cited By 0

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85068184204&doi=10.1016%2fj.scitotenv.2019.06.029&partnerID=40&md5=b8c44177a076e8761d32aab5fe2ca077
DOI10.1016/j.scitotenv.2019.06.029
Citation KeySantangeli2019