Sorry, you need to enable JavaScript to visit this website.

Single cell oil production from undetoxified Arundo donax L. Hydrolysate by cutaneotrichosporon curvatus

TitleSingle cell oil production from undetoxified Arundo donax L. Hydrolysate by cutaneotrichosporon curvatus
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2019
Authorsdi Fidio, N., Liuzzi F., Mastrolitti S., Albergo R., and de Bari I.
JournalJournal of Microbiology and Biotechnology
Volume29
Pagination256-267
ISSN10177825
Keywordsarticle, Arundo donax, Basidiomycetes, Basidiomycota, Biodiesel, biofuel, biofuels, Biomass, Bioreactor, Bioreactors, biosynthesis, biotransformation, Chemical composition, chemistry, Conservation of Natural Resources, controlled study, Culture media, culture medium, Cutaneotrichosporon curvatus, development and aging, Economics, environmental protection, fatty acid, fatty acid analysis, Fatty acids, fed batch fermentation, Fermentation, Glucose, growth, Hydrolysis, Lignin, Lignocellulose, linoleic acid, lipid, Lipids, mathematical model, metabolism, microbiology, Nitrogen, nonhuman, oleic acid, palmitic acid, Poaceae, primary production (biomass), Process optimization, protein hydrolysate, response surface method, single cell analysis, Trichosporon, vegetable oil, xylose
Abstract

The use of low-cost substrates represents one key issue to make single cell oil production sustainable. Among low-input crops, Arundo donax L. is a perennial herbaceous rhizomatous grass containing both C5 and C6 carbohydrates. The scope of the present work was to investigate and optimize the production of lipids by the oleaginous yeast Cutaneotrichosporon curvatus from undetoxified lignocellulosic hydrolysates of steam-pretreated A. donax. The growth of C. curvatus was first optimized in synthetic media, similar in terms of sugar concentration to hydrolysates, by applying the response surface methodology (RSM) analysis. Then the bioconversion of undetoxified hydrolysates was investigated. A fed-batch process for the fermentation of A. donax hydrolysates was finally implemented in a 2-L bioreactor. Under optimized conditions, the total lipid content was 64% of the dry cell weight and the lipid yield was 63% of the theoretical. The fatty acid profile of C. curvatus triglycerides contained 27% palmitic acid, 33% oleic acid and 32% linoleic acid. These results proved the potential of lipid production from A. donax, which is particularly important for their consideration as substitutes for vegetable oils in many applications such as biodiesel or bioplastics. © 2019 by The Korean Society for Microbiology and Biotechnology.

Notes

cited By 0

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85062854341&doi=10.4014%2fjmb.1808.08015&partnerID=40&md5=cf4643afa806ab5bd5f8ba77abd925f3
DOI10.4014/jmb.1808.08015
Citation KeydiFidio2019256