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Molecular and biochemical characterization of a potato collection with contrasting tuber carotenoid content

TitleMolecular and biochemical characterization of a potato collection with contrasting tuber carotenoid content
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2017
AuthorsSulli, Maria, Mandolino G., Sturaro M., Onofri C., Diretto Gianfranco, Parisi B., and Giuliano Giovanni
JournalPLoS ONE
Volume12
Paginatione0184143
ISSN19326203
Keywordsallele, Alleles, article, bioinformatics, Carotenoid, Carotenoids, CHY2 gene, cluster analysis, Correlation analysis, Diploidy, dormancy, epoxy xanthophyll, Gene expression, gene expression profiling, gene expression regulation, gene locus, gene regulatory network, Gene Regulatory Networks, Genes, Genetic Association Studies, genetic association study, genetics, genotype, Glucose, glycolipid, homozygosity, LCYb gene, LCYe gene, liquid chromatography-mass spectrometry, metabolism, metabolome, metabolomics, monogalactosyldiacyglycerol, nonhuman, PDS gene, Phenotype, Plant, plant gene, plant taxonomy, plant tuber, Plant Tubers, Ploidy, potato, procedures, PSY1 gene, PSY2 gene, quantitative analysis, Quantitative Trait Loci, quantitative trait locus, real time polymerase chain reaction, recessive allele, Solanum, Solanum chacoense, Solanum phureja, Solanum tuberosum, suberin, tetraploidy, unclassified drug, xanthophyll, xanthophyll ester derivative, Xanthophylls, ZEP gene
Abstract

After wheat and rice, potato is the third most important staple food worldwide. A collection of ten tetraploid (Solanum tuberosum) and diploid (S. phureja and S. chacoense) genotypes with contrasting carotenoid content was subjected to molecular characterization with respect to candidate carotenoid loci and metabolic profiling using LC-HRMS. Irrespective of ploidy and taxonomy, tubers of these genotypes fell into three groups: yellow-fleshed, characterized by high levels of epoxy-xanthophylls and xanthophyll esters and by the presence of at least one copy of a dominant allele of the β-Carotene Hydroxylase 2 (CHY2) gene; white-fleshed, characterized by low carotenoid levels and by the presence of recessive chy2 alleles; and orange-fleshed, characterized by high levels of zeaxanthin but low levels of xanthophyll esters, and homozygosity for a Zeaxanthin Epoxidase (ZEP) recessive allele. Novel CHY2 and ZEP alleles were identified in the collection. Multivariate analysis identified several groups of co-regulated non-polar compounds, and resulted in the grouping of the genotypes according to flesh color, suggesting that extensive cross-talk exists between the carotenoid pathway and other metabolite pathways in tubers. Postharvest traits like tuber dormancy and weight loss during storage showed little correlation with tuber carotenoid content, with the exception of zeaxanthin and its esters. Other tuber metabolites, such as glucose, monogalactosyldiacyglycerol (a glycolipid), or suberin precursors, showed instead significant correlations with both traits. © 2017 Sulli et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85029389223&doi=10.1371%2fjournal.pone.0184143&partnerID=40&md5=d5ca50edf4c43e23711fad3d9a75baff
DOI10.1371/journal.pone.0184143
Citation KeySulli2017