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Transcriptome analysis in tissue sectors with contrasting crocins accumulation provides novel insights into apocarotenoid biosynthesis and regulation during chromoplast biogenesis

TitleTranscriptome analysis in tissue sectors with contrasting crocins accumulation provides novel insights into apocarotenoid biosynthesis and regulation during chromoplast biogenesis
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2018
AuthorsAhrazem, O., Argandoña J., Fiore Alessia, Aguado C., Luján R., Rubio-Moraga A., Marro M., Araujo-Andrade C., Loza-Alvarez P., Diretto Gianfranco, and Gomez-Gomez L.
JournalScientific Reports
Volume8
ISSN20452322
Keywordsantibody specificity, biosynthesis, Carotenoid, Carotenoids, crocin, Crocus, development and aging, Developmental, gene expression profiling, gene expression regulation, genetics, growth, high throughput sequencing, High-Throughput Nucleotide Sequencing, metabolism, Organ Specificity, Plant, plant protein, Plant Proteins, plastid, Plastids, procedures, RNA, secondary metabolism, Sequence Analysis
Abstract

Crocins, the red soluble apocarotenoids of saffron, accumulate in the flowers of Crocus species in a developmental and tissue-specific manner. In Crocus sieberi, crocins accumulate in stigmas but also in a distinct yellow tepal sector, which we demonstrate contains chromoplast converted from amyloplasts. Secondary metabolites were analysed by LC-DAD-HRMS, revealing the progressive accumulation of crocetin and crocins in the yellow sector, which were also localized in situ by Raman microspectroscopy. To understand the underlying mechanisms of crocin biosynthesis, we sequenced the C. sieberi tepal transcriptome of two differentially pigmented sectors (yellow and white) at two developmental stages (6 and 8) by Illumina sequencing. A total of 154 million high-quality reads were generated and assembled into 248,099 transcripts. Differentially expressed gene analysis resulted in the identification of several potential candidate genes involved in crocin metabolism and regulation. The results provide a first profile of the molecular events related to the dynamics of crocetin and crocin accumulation during tepal development, and present new information concerning apocarotenoid biosynthesis regulators and their accumulation in Crocus. Further, reveals genes that were previously unknown to affect crocin formation, which could be used to improve crocin accumulation in Crocus plants and the commercial quality of saffron spice. © 2018 The Author(s).

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85041946061&doi=10.1038%2fs41598-018-21225-z&partnerID=40&md5=d0abdd6bf171726d2a0611d16711b00b
DOI10.1038/s41598-018-21225-z
Citation KeyAhrazem2018