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The bean polygalacturonase-inhibiting protein 2 (PvPGIP2) is highly conserved in common bean (Phaseolus vulgaris L.) germplasm and related species

TitleThe bean polygalacturonase-inhibiting protein 2 (PvPGIP2) is highly conserved in common bean (Phaseolus vulgaris L.) germplasm and related species
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2009
AuthorsFarina, Anna, Rocchi V., Janni M., Benedettelli S., De Lorenzo G., and D'Ovidio R.
JournalTheoretical and Applied Genetics
Volume118
Pagination1371-1379
ISSN00405752
KeywordsAmino Acid, Amino Acid Sequence, amino acid substitution, Amino acids, animal, Animals, article, bean, classification, Common beans, enzyme inhibitor, Enzyme Inhibitors, enzymology, Extracellular, Gene families, genetics, Germplasm, Inhibitory activities, isoenzyme, Isoenzymes, Leucine-rich repeats, molecular genetics, Molecular Sequence Data, Nicotiana benthamiana, PGIP protein, Phaseolus, Phaseolus vulgaris, Phylogenetic relationships, Phylogeny, Plant, Plant Proteins, Polygalacturonase-inhibiting proteins, Polymorphic sites, Potato virus X, Protein families, Proteins, Sequence Alignment, Sequence comparisons, Sequence conservations, sequence homology, Specific sites, Structural features, vegetable protein, Vulgaris
Abstract

Polygalacturonase-inhibiting proteins (PGIPs) are extracellular plant protein inhibitors of endo-polygalacturonases (PGs) that belong to the leucine-rich repeat (LRR) protein family. In bean, PGIP is encoded by a small gene family of four members among which Pvpgip2 encodes the most wide-spectrum and efficient inhibitor of fungal PGs. In order to evaluate the sequence polymorphism of Pvpgip2 and its functional significance, we have analyzed a number of wild and cultivated bean (P. vulgaris) accessions of Andean and Mesoamerican origin, and some genotypes from the related species P. coccineus, P. acutifolius, and P. lunatus. Our analyses indicate that the protein encoded by Pvpgip2 is highly conserved in the bean germplasm. The few detected polymorphic sites correspond to synonymous substitutions and only two wild genotypes contain a Pvpgip2 with a single non-synonymous replacement. Sequence comparison showed a slightly larger variation in the related bean species P. coccineus, P. acutifolius, and P. lunatus and confirmed the known phylogenetic relationships with P. vulgaris. The majority of the replacements were within the xxLxLxx region of the leucine rich repeat (LRR) domain and none of them affected residues contributing to structural features. The variant PGIPs were expressed in Nicotiana benthamiana using PVX as vector and their inhibitory activity compared to that of PvPPGIP2. All the variants were able to fully inhibit the four fungal PGs tested with minor differences. Taken together these results support the hypothesis that the overall sequence conservation of PGIP2 and minor variation at specific sites is necessary for high-affinity recognition of different fungal PGs. © 2009 Springer-Verlag.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-67349109393&doi=10.1007%2fs00122-009-0987-4&partnerID=40&md5=bfb7339723155a653dee84b7bd2b353a
DOI10.1007/s00122-009-0987-4
Citation KeyFarina20091371