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Micronuclei and 3AB index in X-irradiated human lymphocytes in G1 and G1 phases

TitleMicronuclei and 3AB index in X-irradiated human lymphocytes in G1 and G1 phases
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication1994
AuthorsCatena, C., Villani Paola, Conti D., and Righi E.
JournalMutation Research Regular Papers
Volume311
Pagination231-237
ISSN00275107
Keywords3 aminobenzamide, adult, article, Benzamides, cell cycle g phase, controlled study, DNA repair, Dose-Response Relationship, G0 Phase, G1 Phase, human, interphase, lymphocyte, Lymphocytes, micronucleus, micronucleus test, Micronucleus Tests, Middle Age, Non-U.S. Gov't, priority journal, Radiation, Radiation Tolerance, Radiation-Sensitizing Agents, Support, Variation (Genetics), X ray
Abstract

We applied the cytokinesis-block micronucleus assay to observe the radiobiological response of human lymphocytes after X-ray treatment in the G0 and G1 phases. In addition, we used 3-aminobenzamide (3AB) to measure the 3AB index in the two phases. The experimental results show that the at 2 Gy the MN yield and the 3AB index are dependent on the cell phase and show considerable inter-individual variability. The radiation-induced MN frequency obtained for 33 subjects is 0.470 ± 0.063 for the G0 phase and 0.689 ± 0.139 for the G1 phase; the 3AB index values are 0.326 ± 0.144 and 0.067 ± 0.058 for G0 and G1 phases, respectively. At the individual level, the 3AB index for the G1 phase correlations inversely with the cytogenetic effects observed in that phase. We discuss the possibility of applying the MN test combined with the 3AB index to lymphocytes at different phases to study the individual response to radiation (individual radiosensitivity). © 1994.

Notes

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0027973125&doi=10.1016%2f0027-5107%2894%2990181-3&partnerID=40&md5=3221387fb97a66fbd9ff9f5c63d99cdf
DOI10.1016/0027-5107(94)90181-3
Citation KeyCatena1994231