| Title | Krill oil reduces intestinal inflammation by improving epithelial integrity and impairing adherent-invasive Escherichia coli pathogenicity |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2016 |
| Authors | Costanzo, Manuela, Cesi Vincenzo, Prete E., Negroni Anna, Palone Francesca, Cucchiara S., Oliva S., Leter B., and Stronati L. |
| Journal | Digestive and Liver Disease |
| Volume | 48 |
| Pagination | 34-42 |
| ISSN | 15908658 |
| Keywords | actin, Actins, adherent invasive Escherichia coli, animal, animal cell, Animals, antiinflammatory agent, article, Bacterial Adhesion, bacterial strain, bacterium adherence, CACO 2 cell line, Caco-2 cell line, Caco-2 Cells, cadherin, Cadherins, cell adhesion, cell death, cell invasion, cell line RAW 264.7, Cell Survival, controlled study, down regulation, drug effects, enteritis, Escherichia coli, Escherichia coli infection, Escherichia coli Infections, Euphausiacea, F actin, Fatty acids, fish oil, Flow cytometry, gamma interferon, genetics, HT 29 cell line, HT-29 cell line, HT29 Cells, human, human cell, Humans, Immunofluorescence, immunology, Interferon-gamma, interleukin 8, Interleukin-8, intestine epithelium cell, krill, krill oil, macrophage, Messenger, messenger RNA, metabolism, Mice, microbial viability, mouse, nonhuman, omega 3 fatty acid, Omega-3, pathogenesis, Pathogenicity, physiology, priority journal, protein expression, protein ZO1, RAW 264.7 cell line, RAW 264.7 Cells, RNA, TJP1 protein, Tumor Necrosis Factor, tumor necrosis factor alpha, Tumor Necrosis Factor-alpha, unclassified drug, uvomorulin, wound healing, Zonula Occludens-1 Protein |
| Abstract | Background: Krill oil is a marine derived oil rich in phospholipids, astaxanthin and omega-3 fatty acids. Several studies have found benefits of krill oil against oxidative and inflammatory damage. Aims: We aimed at assessing the ability of krill oil to reduce intestinal inflammation by improving epithelial barrier integrity, increasing cell survival and reducing pathogenicity of adherent-invasive Escherichia coli. Methods: CACO2 and HT29 cells were exposed to cytomix (TNFα and IFNγ) to induce inflammation and co-exposed to cytomix and krill oil. E-cadherin, ZO-1 and F-actin levels were analyzed by immunofluorescence to assess barrier integrity. Scratch test was performed to measure wound healing. Cell survival was analyzed by flow cytometry. Adherent-invasive Escherichia coli LF82 was used for adhesion/invasion assay. Results: In inflamed cells E-cadherin and ZO-1 decreased, with loss of cell-cell adhesion, and F-actin polymerization increased stress fibres; krill oil restored initial conditions and improved wound healing, reduced bacterial adhesion/invasion in epithelial cells and survival within macrophages; krill oil reduced LF82-induced mRNA expression of pro-inflammatory cytokines. Conclusions: Krill oil improves intestinal barrier integrity and epithelial restitution during inflammation and controls bacterial adhesion and invasion to epithelial cells. Thus, krill oil may represent an innovative tool to reduce intestinal inflammation. © 2015 Editrice Gastroenterologica Italiana S.r.l. |
| Notes | cited By 7 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955729478&doi=10.1016%2fj.dld.2015.09.012&partnerID=40&md5=ffbd5ced64e3fac8711b78bc8ebafb58 |
| DOI | 10.1016/j.dld.2015.09.012 |
| Citation Key | Costanzo201634 |
