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Fifty-Hertz Magnetic Field Affects the Epigenetic Modulation of the miR-34b/c in Neuronal Cells.

TitleFifty-Hertz Magnetic Field Affects the Epigenetic Modulation of the miR-34b/c in Neuronal Cells.
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2018
AuthorsConsales, Claudia, Cirotti Claudia, Filomeni Giuseppe, Panatta Martina, Butera Alessio, Merla Caterina, Lopresto Vanni, Pinto Rosanna, Marino Carmela, and Benassi Barbara
JournalMol Neurobiol
Volume55
Issue7
Pagination5698-5714
Date Published2018 Jul
ISSN15591182
Abstract

The exposure to extremely low-frequency magnetic fields (ELF-MFs) has been associated to increased risk of neurodegenerative diseases, although the underlying molecular mechanisms are still undefined. Since epigenetic modulation has been recently encountered among the key events leading to neuronal degeneration, we here aimed at assessing if the control of gene expression mediated by miRNAs, namely miRs-34, has any roles in driving neuronal cell response to 50-Hz (1 mT) magnetic field in vitro. We demonstrate that ELF-MFs drive an early reduction of the expression level of miR-34b and miR-34c in SH-SY5Y human neuroblastoma cells, as well as in mouse primary cortical neurons, by affecting the transcription of the common pri-miR-34. This modulation is not p53 dependent, but attributable to the hyper-methylation of the CpG island mapping within the miR-34b/c promoter. Incubation with N-acetyl-l-cysteine or glutathione ethyl-ester fails to restore miR-34b/c expression, suggesting that miRs-34 are not responsive to ELF-MF-induced oxidative stress. By contrast, we show that miRs-34 control reactive oxygen species production and affect mitochondrial oxidative stress triggered by ELF-MFs, likely by modulating mitochondria-related miR-34 targets identified by in silico analysis. We finally demonstrate that ELF-MFs alter the expression of the α-synuclein, which is specifically stimulated upon ELF-MFs exposure via both direct miR-34 targeting and oxidative stress. Altogether, our data highlight the potential of the ELF-MFs to tune redox homeostasis and epigenetic control of gene expression in vitro and shed light on the possible mechanism(s) producing detrimental effects and predisposing neurons to degeneration.

DOI10.1007/s12035-017-0791-0
Alternate JournalMol. Neurobiol.
Citation Key6750
PubMed ID29039021
Grant ListR72-A4647 / / Kræftens Bekæmpelse (DK) /
R146-A9414 / / Kræftens Bekæmpelse (DK) /
AIRC-MFAG 2011 n.1145 / / Associazione Italiana per la Ricerca sul Cancro /