Extraction of astaxanthin from microalga Haematococcus pluvialis in red phase by using generally recognized as safe solvents and accelerated extraction

TitleExtraction of astaxanthin from microalga Haematococcus pluvialis in red phase by using generally recognized as safe solvents and accelerated extraction
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2018
AuthorsMolino, Antonio, Rimauro Juri, Casella P., Cerbone A., Larocca Vincenzo, Chianese S., Karatza D., Mehariya S., Ferraro A., Hristoforou E., and Musmarra D.
JournalJournal of Biotechnology
Volume283
Pagination51-61
ISSN01681656
KeywordsAccelerated solvent extraction, Acetone, Antioxidants, Astaxanthin, Bioprocesses, Effect of temperature, Extraction temperatures, Haematococcus pluvialis, Investigating effects, Isomers, Mechanical pre-treatment, Organic solvents, Recovery, Solvent extraction
Abstract

Solvent Extraction was tested to extract astaxanthin from Haematococcus pluvialis in red phase (HPR), by investigating effects of solvents, extraction pressure and temperature. Astaxanthin isomers were identified and quantified in the extract. The performances of acetone and ethanol, Generally Recognized As Safe (GRAS) solvents, were explored. Negligible effect of pressure was found, while with increasing extraction temperature astaxanthin recovery increased till a maximum value, beyond which thermal degradation seemed to be greater than the positive effect of temperature on extraction. Furthermore, to maximize the extraction yield of astaxanthin, mechanical pre-treatment of HPR biomass was carried out and several extraction runs were consecutively performed. Experimental results showed that after the mechanical pre-treatment the astaxanthin recovery strongly increased while a single extraction run of 20 min was sufficient to extract more than 99% of total astaxanthin extracted. After pre-treatment, maximum recovery of about 87% was found for acetone (pressure = 100 bar; temperature = 40 °C; total time = 60 min). © 2018

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85050212521&doi=10.1016%2fj.jbiotec.2018.07.010&partnerID=40&md5=895d5a83b786c365315adb9c57b2a5c6
DOI10.1016/j.jbiotec.2018.07.010