| Title | Frequency spectrum of induced transmembrane potential and permeabilization efficacy of bipolar electric pulses |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2017 |
| Authors | Merla, Caterina, Pakhomov A.G., Semenov I., and Vernier P.T. |
| Journal | Biochimica et Biophysica Acta - Biomembranes |
| Volume | 1859 |
| Pagination | 1282-1290 |
| ISSN | 00052736 |
| Keywords | animal, animal cell, Animals, article, Biological, biological model, bipolar electric pulse, calcium transport, Cell Membrane Permeability, CHO cell line, CHO Cells, Cricetinae, Cricetulus, Culture media, culture medium, Electricity, electroporation, Fourier analysis, hamster, mathematical computing, membrane potential, Membrane Potentials, microdosimetry, Models, nonhuman, signal processing, Spectroscopy, spectrum |
| Abstract | In this paper a simple prediction method for the bipolar pulse cancellation effect is proposed, based on the frequency analysis of the TMP spectra of a single cell and the computed relative global spectral content up to a defined frequency threshold. We present a spectral analysis of pulses applied in experiments, and we extract the induced TMP from a microdosimetric model of the cell. The induced TMP computation is carried out on a hemispherical multi-layered cell model in the time domain. The analysis is presented for a variety of unipolar and bipolar input signals in the nanosecond and the microsecond time scales. Our evaluations are in good agreement with experimental results for bipolar pulse cancellation of electropermeabilization-induced Ca2+ influx using 300 ns, 750 kV/m pulses and with other results reported in recent literature. © 2017 Elsevier B.V. |
| Notes | cited By 1 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018741550&doi=10.1016%2fj.bbamem.2017.04.014&partnerID=40&md5=ac2641283fafe5a38fad42fed51bf91e |
| DOI | 10.1016/j.bbamem.2017.04.014 |
| Citation Key | Merla20171282 |
