|Title||DNA fragmentation induced by Fe ions in human cells: Shielding influence on spatially correlated damage|
|Publication Type||Articolo su Rivista peer-reviewed|
|Year of Publication||2004|
|Authors||Antonelli, Francesca, Belli M., Campa A., Chatterjee A., Dini V., Esposito G., Rydberg B., Simone G., and Tabocchini M.A.|
|Journal||Advances in Space Research|
|Keywords||article, Astronomy, Cell Line, Cells, Cosmic rays, DNA, DNA damage, DNA fragment, DNA fragmentation, Dose-Response Relationship, dosimetry, evaluation, Evaluation Studies, Extraterrestrial Environment, Fe ions in human cells, fibroblast, Fibroblasts, gamma radiation, Gamma rays, heavy ion, Heavy Ions, human, Humans, instrumentation, Iron, linear energy transfer, methodology, microclimate, NASA Discipline Radiation Health, Non-NASA Center, poly(methyl methacrylate), Polymethyl Methacrylate, Radiation, Radiation exposure, radiation protection, radiation response, Radiation risk, Radiation shielding, relative biologic effectiveness, Relative Biological Effectiveness, Risk assessment, synchrotron, Synchrotrons|
Outside the magnetic field of the Earth, high energy heavy ions constitute a relevant part of the biologically significant dose to astronauts during the very long travels through space. The typical pattern of energy deposition in the matter by heavy ions on the microscopic scale is believed to produce spatially correlated damage in the DNA which is critical for radiobiological effects. We have investigated the influence of a lucite shielding on the initial production of very small DNA fragments in human fibroblasts irradiated with 1 GeV/u iron (Fe) ions. We also used γ-rays as reference radiation. Our results show: (1) a lower effect per incident ion when the shielding is used; (2) an higher DNA Double Strand Breaks (DSB) induction by Fe ions than by γ-rays in the size range 1-23 kbp; (3) a non-random DNA DSB induction by Fe ions. © 2004 COSPAR. Published by Elsevier Ltd. All rights reserved.
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