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DNA DSB induced by iron ions in human fibroblasts: LET dependence and shielding efficiency

TitleDNA DSB induced by iron ions in human fibroblasts: LET dependence and shielding efficiency
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2005
AuthorsEsposito, G., Antonelli Francesca, Belli M., Campa A., Dini V., Furusawa Y., Simone G., Sorrentino E., and Tabocchini M.A.
JournalAdvances in Space Research
KeywordsAluminum, article, cell culture, Cell Line, Charged particles, comparative study, cosmic radiation, DNA, DNA damage, DNA DSB, Electrophoresis, fibroblast, Fibroblasts, heavy ion, Heavy Ions, High charge and energy (HZE) particles, human, Humans, Ion bombardment, Ionizing radiation, Iron, Lead, linear energy transfer, poly(methyl methacrylate), Polymethyl Methacrylate, Radiation Dosage, radiation dose, Radiation exposure, radiation protection, Radiation shielding, RBE-LET relationship, relative biologic effectiveness, Relative Biological Effectiveness, Space radiation, synchrotron, Synchrotrons

This paper reports on DNA DSB induction in human fibroblasts by iron ions of different energies, namely 5, 1 GeV/u, 414 and 115 MeV/u, in absence or presence of different shields (PMMA, Al and Pb). Measure of DNA DSB was performed by calibrated Pulsed Field Gel Electrophoresis using the fragment counting method. The RBE-LET relationships for unshielded and shielded beams were obtained both in terms of dose average LET and of track average LET. Weak dependence on these parameters was observed for DSB induction. The shielding efficiency, evaluated by the ratio between the cross sections for unshielded and shielded beams, depends not only on the shield type and thickness, but also on the beam energy. Protection is only observed at high iron ions energy, especially at 5 GeV/u, where PMMA shield gives higher protection compared to Al or Pb shields of the same thickness expressed in g/cm2. © 2004 COSPAR. Published by Elsevier Ltd. All rights reserved.


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Citation KeyEsposito2005243