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Chimeric plant virus particles as immunogens for inducing murine and human immune responses against human immunodeficiency virus type 1

TitleChimeric plant virus particles as immunogens for inducing murine and human immune responses against human immunodeficiency virus type 1
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2001
AuthorsMarusic, Carla, Rizza P., Lattanzi L., Mancini C., Spada M., Belardelli F., Benvenuto Eugenio, and Capone I.
JournalJournal of Virology
KeywordsAIDS Vaccines, Amino Acid Sequence, animal experiment, animal model, Animals, antibody response, article, B-Lymphocyte, Capsid, Capsid Proteins, chimeric protein, coat protein, controlled study, dendritic cell, epitope, Epitopes, Female, Genetic engineering, Genetic Vectors, glycoprotein gp 41, HIV Envelope Protein gp41, HIV Infections, HIV-1, human, human cell, Human immunodeficiency virus 1, Human immunodeficiency virus infection, Human immunodeficiency virus vaccine, Humans, immune response, immunization, immunogenicity, immunoglobulin A antibody, immunoglobulin G antibody, Inbred C57BL, Mice, Molecular Sequence Data, mouse, nonhuman, peripheral lymphocyte, plant virus, Potexvirus, priority journal, Recombinant Fusion Proteins, SCID, SCID mouse, Synthetic, Vaccines, Virion, virus antigen, virus neutralization, virus particle

The high-yield expression of a neutralizing epitope from human immunodeficiency virus type 1 (HIV-1) on the surface of a plant virus and its immunogenicity are presented. The highly conserved ELDKWA epitope from glycoprotein (gp) 41 was expressed as an N-terminal translational fusion with the potato virus X (PVX) coat protein. The resulting chimeric virus particles (CVPs), purified and used to immunize mice intraperitoneally or intranasally, were able to elicit high levels of HIV-1-specific immunoglobulin G (IgG) and IgA antibodies. Furthermore, the human immune response to CVPs was studied with severe combined immunodeficient mice reconstituted with human peripheral blood lymphocytes (hu-PBL-SCID). hu-PBL-SCID mice immunized with CVP-pulsed autologous dendritic cells were able to mount a specific human primary antibody response against the gp41-derived epitope. Notably, sera from both normal and hu-PBL-SCID mice showed an anti-HIV-1-neutralizing activity. Thus, PVX-based CVPs carrying neutralizing epitopes can offer novel perspectives for the development of effective vaccines against HIV and, more generally, for the design of new vaccination strategies in humans.


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Citation KeyMarusic20018434