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Immunomodulatory activity of a plant extract containing human papillomavirus 16-E7 protein in human monocyte-derived dendritic cells

TitleImmunomodulatory activity of a plant extract containing human papillomavirus 16-E7 protein in human monocyte-derived dendritic cells
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2009
AuthorsDi Bonito, P., Grasso F., Mangino G., Massa Silvia, Illiano E., Franconi Rosella, Fanales-Belasio E., Falchi M., Affabris E., and Giorgi C.
JournalInternational Journal of Immunopathology and Pharmacology
KeywordsAdjuvants, antigen presentation, article, biological marker, cancer immunotherapy, cell differentiation, Cell Line, cell maturation, Cell Survival, Coculture Techniques, Cytotoxicity, dendritic cell, Dendritic Cells, Dose-Response Relationship, Drug, drug cytotoxicity, drug effect, drug mixture, drug screening, expression vector, Female, fluorescein isothiocyanate, Genetic Vectors, Genetically Modified, human, human cell, Human papillomavirus type 16, Humans, Immunologic, Immunomodulation, lymphocyte, lymphocyte activation, Lymphocytes, male, medicinal plant, monocyte, Nicotiana bentamiana, Nicotiana bentamiana extract, nonhuman, Oncogene Proteins, ovalbumin, Papillomavirus Vaccines, peripheral blood mononuclear cell, Phenotype, Plant extract, Plant extracts, plant leaf, Plant leaves, Plants, Potexvirus, priority journal, protein E7, Recombinant Proteins, Time Factors, Tobacco, Tumor, tumor vaccine, unclassified drug, Viral

This study reports the immunomodulatory activity on human Monocyte Derived Dendritic Cells (MDDCs) of a vaccine preparation shown to be effective against an HPV16-related tumour in an animal model. The vaccine is composed of extract from Nicotiana benthamiana leaves containing HPV16 E7 protein expressed by a potato virus X-derived vector (NbPVX-E7). The effect of the extract was evaluated on MDDC differentiation and maturation by monitoring the phenotypic expression of specific markers. The results show that NbPVX-E7 does not induce monocyte differentiation to dendritic cells, but does induce MDDC maturation. Plant extract does not influence MDDC-uptake of E7-FITC while it significantly improves the Ovalbumin-FITC uptake, considered as a model antigen. Importantly, NbPVX-E7-pulsed MDDCs/PBMCs are able to prime human blood-derived lymphocytes from healthy individuals to induce HPV16 E7-specific cytotoxic activity. This is a propaedeutic study for a possible use of E7-containing plant extract in human immunotherapy of HPV-related lesions. Copyright © by Biolife, s.a.s.


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Citation KeyDiBonito2009967