Influence of mediator immobilization on the electrochemically assisted microbial dechlorination of trichloroethene (TCE)and cis-dichloroethene (cis-DCE)

TitleInfluence of mediator immobilization on the electrochemically assisted microbial dechlorination of trichloroethene (TCE)and cis-dichloroethene (cis-DCE)
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2009
AuthorsAulenta, F., Canosa A., De Roma L., Reale P., Panero S., Rossetti S., and Majone M.
JournalJournal of Chemical Technology and Biotechnology
Keywordsarticle, bacterium culture, Biocathode, Bioelectrochemical systems, chemical modification, controlled study, dechlorination, Dehalococcoides, Dehalococcoides spp., Desulfitobacterium, Dissolution, Electrochemistry, electrode, Electrodes, Ethylene, Hydrogen, Immobilization, Methyl viologen, Microbial electrodes, nonhuman, oxidation reduction reaction, paraquat, pH electrode, Reaction kinetics, Reduction, Reductive dechlorination, solid state, Trichloroethene, Trichloroethylene

Background: A bioelectrochemical process for trichloroethene (TCE) dechlorination was developed. In this new process, a solid-state electrode polarized to-450 mV versus the standard hydrogen electrode (SHE), in combination with a redox mediator (i.e., methyl viologen, MV) is employed as an electron donor for the microbial reductive dechlorination of TCE. In this study we compared the performance of the process with the redox mediator immobilized at the surface of electrodes or dissolved in the bulk liquid, using both a culture highly enriched in Desulfitobacterium spp., capable of dechlorinating TCE to cis-dichloroethene (cis-DCE), and a culture highly enriched in Dehalococcoides spp. capable of dechlorinating cis-DCE to ethene. Results: Short-term potentiostatic (-450 mV versus SHE) experiments showed that TCE or cis-DCE was dechlorinated both in the presence of soluble (500 μmol L-1) and immobilized MV. However, TCE or cis-DCE dechlorination rates with MV-modified electrodes were remarkably lower than with soluble MV. Both cultures produced significant amounts of H2 in the presence of electrically reduced, soluble MV, whereas no H2 was produced when the mediator was immobilized at the electrode surface, regardless of the potential applied to the electrode, in the range -425 to -500 mV versus SHE. Conclusions: The process, operated with modified electrodes, supports the microbial dechlorination of TCE to ethene. Immobilization not only allows retention of the mediator within the system, but also increases process efficiency by preventing bioelectrochemical H2 formation. On the other hand, strategies to increase dechlorination rates with modified electrodes need to be developed. © 2009 Society of Chemical Industry.


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