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Suitability of lysosomal membrane stability in Eisenia fetida as biomarker of soil copper contamination

TitleSuitability of lysosomal membrane stability in Eisenia fetida as biomarker of soil copper contamination
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2011
AuthorsRocco, A., Scott-Fordsmand J.J., Maisto G., Manzo Sonia, Salluzzo Antonio, and Jensen J.
JournalEcotoxicology and Environmental Safety
Volume74
Pagination984-988
ISSN01476513
KeywordsAnimals, article, bioaccumulation, biological marker, Biological Markers, biomarker, concentration (composition), concentration (parameters), controlled study, Copper, earthworm, Eisenia fetida, environmental exposure, Environmental monitoring, Intracellular Membranes, lysosome membrane stability, Lysosomes, neutral red, nonhuman, Oligochaeta, pollution effect, pollution exposure, pollution monitoring, Soil, Soil Pollutants, Soil pollution
Abstract

Accumulated metals in soils negatively affect dwelling organisms. Earthworms, which are widespread and perform various essential functions, are able to accumulate metals that can damage the coelomic cells. The aim of this work was to evaluate the effect on Eisenia fetida lysosomal membrane stability both during and after copper exposure, and finally to link this to internal concentrations. E. fetida specimens were exposed to a reference soil and two Cu-spiked soils (35 and 350mgkg-1d.w.) for 14 days (uptake period) and then transferred into the reference soil for other 18 days (elimination period). After 3 days of uptake, internal Cu concentrations increased and were higher in the specimens exposed to soils spiked with 350mgCukg-1 d.w. After 2 days of elimination, a strong decrease of internal Cu concentrations was always observed. The lysosomal membrane stability, measured as neutral red retention-times, was approximately 50min for the earthworms exposed to the reference soils, whereas it decreased, at the end of the uptake period, to 21 and 13min, respectively, for the organisms exposed to soils spiked with 35 and 350mgCukg-1 d.w. A full recovery of the lysosomal membrane stability was reached after 14 and 18 days of the elimination period, respectively, for the organisms exposed to soils spiked with 35 and 350mgCukg-1 d.w. The neutral-red assay would seem a good biomarker since the lysosomal membrane stability of E. fetida appeared to respond rapidly and strongly to soil copper contamination. © 2011 Elsevier Inc.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-79955523547&doi=10.1016%2fj.ecoenv.2011.01.013&partnerID=40&md5=3118579cfd928887b806c8580bcd775d
DOI10.1016/j.ecoenv.2011.01.013
Citation KeyRocco2011984