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Amniotic epithelial stem cell biocompatibility for electrospun poly(lactide-co-glycolide), poly(ε-caprolactone), poly(lactic acid) scaffolds

TitleAmniotic epithelial stem cell biocompatibility for electrospun poly(lactide-co-glycolide), poly(ε-caprolactone), poly(lactic acid) scaffolds
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2016
AuthorsRusso, V., Tammaro Loredana, Di Marcantonio L., Sorrentino A., Ancora M., Valbonetti L., Turriani M., Martelli A., Cammà C., and Barboni B.
JournalMaterials Science and Engineering C
Volume69
Pagination321-329
ISSN09284931
Keywordsbiocompatibility, Biodegradable polymers, Biodegradable thermoplastics, cell adhesion, Cells, Contact angle, cytology, Electrospinning, Electrospun scaffolds, Fibers, Interconnected pores, Morphological characterization, Organic polymers, Poly (epsiloncaprolactone), Poly lactide-co-glycolide, Pore size, Porosity and pore size, Scaffolds (biology), Scanning electron microscopy, Spatial organization, Stem cells, tissue engineering, X ray diffraction
Abstract

Three biodegradable thermoplastic polymers, poly(ε-caprolactone) (PCL), poly(L-lactide-co-D,L-lactide) (PLA) and poly(L-lactide-co-glycolide) (PLGA), have been used to produce nonwovens scaffolds with uniform micrometer fibres. Scaffolds' physical and morphological characterization was performed by X-ray diffraction, Scanning Electron Microscopy and Contact-Angle test. Morphological investigations revealed that all produced fibres were randomly orientated with interconnected pores ranging between 5 and 12 μm in diameter. An average fibre diameter of 1.5, 0.75 and 1.2 μm was found for PCL, PLA and PLGA, respectively. Moreover, experiments were designed to verify whether the fabricated electrospun substrates were biocompatible for ovine amniotic epithelial stem cells (oAECs) under in vitro conditions. Cell adhesion, survival, spatial organization on fibres, proliferation index, and DNA quantification after 48 h culture, showed an enhanced adhesion and proliferation, especially for PLGA scaffolds. The favourable interaction between oAECs and the fibrous scaffolds was attributed to the greatly improved porosity and pore size distribution of the electrospun scaffolds. In addition, AECs can be considered ideal for tissue engineering especially when using biocompatible and opportunely produced scaffolds. © 2016 Elsevier B.V.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84976889256&doi=10.1016%2fj.msec.2016.06.092&partnerID=40&md5=87a5b05cfca54e73d9866d58b8b67c23
DOI10.1016/j.msec.2016.06.092
Citation KeyRusso2016321