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Growth and adsorption of Streptococcus mutans 6715-13 to hydroxyapatite in the presence of lactoferrin

TitleGrowth and adsorption of Streptococcus mutans 6715-13 to hydroxyapatite in the presence of lactoferrin
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication1989
AuthorsVisca, P., Berlutti F., Vittorioso P., Dalmastri Claudia, Thaller M.C., and Valenti P.
JournalMedical Microbiology and Immunology
Volume178
Pagination69 - 79
Date Published1989///
Abstract

The growth of Streptococcus mutans 6715-13 in a rich medium (Todd Hewitt broth) was drastically reduced by addition of apo-lactoferrin (apo-Lf); this effect was bacteriostatic and reversible by saturation of Lf with iron. The influence of Lf, salivary proteins (SP) and bovine serum albumin (BSA) on the attachment of Streptococcus mutans to hydroxyapatite (HA) was successively investigated. Sorption of Lf, SP, and BSA to HA was dependent on the protein concentration and reached the end-point at about 80 mg of proteins per gram of HA. Similarly, the number of streptococci adsorbed to HA was correlated to the amount of cells available up to at least 107 cells per mg of HA. The adsorption of Lf, SP and BSA on HA reduced the number of attaching S. mutans cells. In particular, SP reduced the adsorption of S. mutans by 30%, whereas pre-coating of HA with apo- or iron-saturated Lf resulted in a three orders of magnitude reduction of S. mutans adsorption to HA, as demonstrated by means of different experimental procedures. The powerful adherence-inhibiting effect of apo-Lf together with its noticeable antibacterial activity towards S. mutans points to a biological significance of these phenomena also in vivo.

Notes

Cited By (since 1996): 9Export Date: 26 August 2010Source: Scopus

URLhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0024511981&partnerID=40&md5=0467fe7773435283c4c9e21c0022ab0a
Citation Key498