| Title | Genetic transformation of potato (Solanum tuberosum): An efficient method to obtain transgenic plants |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 1989 |
| Authors | Tavazza, Raffaela, Tavazza Mario, Ordàs R.J., Ancora Giorgio, and Benvenuto Eugenio |
| Journal | Plant Science |
| Volume | 59 |
| Pagination | 175 - 181 |
| Date Published | 1989/// |
| Keywords | Agrobacterium tumefaciens, binary vector, genetic manipulation, neomycin phosphotransferase, potato, Transformation |
| Abstract | A quick procedure for efficient transformation of potato (cv. Désirée) has been devised. Leaf disc have been inoculated with an Agrobacterium tumefaciens harbouring a Ti plasmid derived binary vector. Transformed shoots carrying the neomycin phosphotransferase gene were regenerated using a feeder layer technique after 4 weeks on selective medium containing kanamycin. Transgenic plants obtained in large numbers appeared phenotypically normal and expressed the NPT II gene. The present work points out that culture conditions are fundamental to obtain the highest transformation efficiency. © 1989. |
| Notes | Cited By (since 1996): 14Export Date: 25 August 2010Source: Scopus |
| URL | http://www.scopus.com/inward/record.url?eid=2-s2.0-45249126456&partnerID=40&md5=435948a95d4602ca524262569cc9c4eb |
| Citation Key | 295 |
