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Peptide display on Potato virus X: molecular features of the coat protein-fused peptide affecting cell-to-cell and phloem movement of chimeric virus particles.

TitlePeptide display on Potato virus X: molecular features of the coat protein-fused peptide affecting cell-to-cell and phloem movement of chimeric virus particles.
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2006
AuthorsLico, Chiara, Capuano Floriana, Renzone Giovanni, Donini Marcello, Marusic Carla, Scaloni Andrea, Benvenuto Eugenio, and Baschieri Selene
JournalThe Journal of general virology
Volume87
Pagination3103-12
Date Published2006 Oct
ISSN0022-1317
KeywordsCapsid Proteins, Genetic engineering, Movement, mutation, Plant leaves, Plasmids, Potexvirus, Recombinant Fusion Proteins, Tobacco
Abstract

The potexvirus Potato virus X (PVX) can be modified genetically to generate chimeric virus particles (CVPs) carrying heterologous peptides fused to coat protein (CP) subunits. A spontaneous PVX mutant expressing a truncated, but functional, form of the CP has been isolated. With the aim of exploiting this virus to display peptides useful for vaccine formulations, two novel viral expression vectors based on pPVX201 (bearing the wild-type PVX genome) were constructed encoding the truncated CP. Both vectors were able to produce infectious virus particles in planta and were used to insert a panel of sequences encoding peptides of biopharmaceutical interest as N-terminal fusions to the truncated cp gene. The analysis of infection progression induced by the different constructs enabled identification of two important structural features of the fused peptide, namely tryptophan content and isoelectric point, critically affecting the formation of PVX CVPs and virus movement through the plant. These results are discussed in view of the rising interest in engineered plant viruses for development of peptide-based epitope vaccines.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-33749065356&doi=10.1099%2fvir.0.82097-0&partnerID=40&md5=10777d2bcca85d21a0aadacc3115f714
DOI10.1099/vir.0.82097-0
Citation Key5411