| Title | Unlocking the potential of Metschnikowia pulcherrima: a dive into the genomic and safety characterization of four plant-associated strains |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2025 |
| Authors | Larini, Ilaria, Ferrara Massimo, Troiano Eleonora, Gatto Veronica, Mulè Giuseppina, Vitulo Nicola, Capozzi Vittorio, Salvetti Elisa, Felis Giovanna E., and Torriani Sandra |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 109 |
| Type of Article | Article |
| ISSN | 01757598 |
| Keywords | Agro foods, alpha glucosidase, alpha mannosidase, Anti-microbial activity, antifungal susceptibility, antimicrobial activity, Antimycotic resistance, Antimycotics, article, biocontrol agent, biogenic amine, bioinformatics, biological pest control, biotechnological production, carbohydrate, carrier protein, computer model, contig, controlled study, DNA base composition, DNA extraction, Drug, drug effect, enzyme activity, enzymology, Fungal, fungal DNA, fungal gene, fungal genome, fungal strain, fungal virulence, fungus growth, genetics, Genome, Genomic analyze, genomic DNA, Genomics, Genomics analysis, glycosidase, glycosyltransferase, in vitro study, itraconazole, Metschnikowia, metschnikowia pulcherrima, nonhuman, Plant, Plant pathogen, polysaccharide, protein localization, pulcherrimin, Quality control, ribosome protein, safety, safety assessment, Safety assessments, signal peptide, sphingosine acyltransferase, unclassified drug, Vegetation, virulence factor, whole genome sequencing, Yeast |
| Abstract | Abstract: Metschnikowia pulcherrima includes strains of applied agro-food interest, particularly due to the antimicrobial activity against plant pathogens, contribution to the aroma of fermented beverages, and preliminary evidence related to probiotic activity. This biotechnological relevance sheds new light of interest on the biology of this yeast. To better understand and expand its biotechnological potential and applicability, the genomes of M. pulcherrima NRRL Y-7111 T, NRRL Y-48695, CBS 10357, and NRRL Y-48712 were sequenced, and de-novo assembled. Between 10,671 and 14,548 genes were predicted and the cooperative genomic analyses were integrated with experimental assessments relating to traits relevant for biotechnological application and safety. In silico and in vitro safety assessment revealed intermediate sensitivity for itraconazole; furthermore, variants of the genes related to pulcherrimin production and transport were found in all the genomes. Moreover, an arsenal of carbohydrate-active enzymes (CAZymes) was unravelled, and their predicted localization was investigated. This study expands the body of knowledge on M. pulcherrima, including traits relevant for defining its safety as a bioresource, which is a pivotal aspect for its possible inclusion in the European Food Safety Authority (EFSA) Qualified Presumption of Safety (QPS) list and its application in REgulated food/feed PROducts (REPRO) both in the European Union & aligned European countries. © The Author(s) 2025. |
| Notes | Cited by: 4; All Open Access; Gold Open Access; Green Open Access |
| URL | https://www.scopus.com/pages/publications/105006891716?origin=resultslist |
| DOI | 10.1007/s00253-025-13515-0 |
| Citation Key | Larini2025 |
| PubMed ID | 40439729 |
