Sorry, you need to enable JavaScript to visit this website.

β-Arrestin-1 inhibits glucocorticoid receptor turnover and alters glucocorticoid signaling

Titleβ-Arrestin-1 inhibits glucocorticoid receptor turnover and alters glucocorticoid signaling
Publication TypeArticolo su Rivista peer-reviewed
Year of Publication2019
AuthorsPetrillo, Maria Grazia, Oakley Robert H., and Cidlowski John A.
JournalJournal of Biological Chemistry
Pagination11225 – 11239
Type of ArticleArticle
KeywordsA-549 cell line, A549 cells, Apoptosis, article, beta arrestin 1, beta-Arrestin 1, binding affinity, Catalysis, cell differentiation, cell proliferation, Cells, Co-immunoprecipitations, controlled study, cytology, cytoplasm, G protein coupled receptors, gene knockdown, Gene Knockdown Techniques, gene silencing, Genetic, genetic transcription, genetics, glucocorticoid, glucocorticoid receptor, Glucocorticoids, Hormones, human, human cell, Humans, Immunoprecipitation, In Situ Hybridization, Inflammatory disease, metabolism, nuclear protein, Nuclear Proteins, PELI1 protein, priority journal, Proteasomal degradation, Protein complexes, protein degradation, protein expression, protein function, protein protein interaction, Protein-protein interactions, Proteins, Receptors, Regulatory protein, Scaffolds (biology), signal transduction, Small interfering RNA, Transcription, ubiquitin protein ligase, ubiquitin protein ligase E3, Ubiquitin-Protein Ligases, ubiquitination, Up-Regulation, upregulation

Glucocorticoids are among the most widely used drugs to treat many autoimmune and inflammatory diseases. Although much research has been focused on investigating glucocorticoid activity, it remains unclearhowglucocorticoids regulate distinct processes in different cells. Glucocorticoids exert their effects through the glucocorticoid receptor (GR), which, upon glucocorticoid binding, interacts with regulatory proteins, affecting its activity and function. These protein-protein interactions are necessary for the resolution of glucocorticoid-dependent physiological and pharmacological processes. In this study, we discovered a novel protein interaction between the glucocorticoid receptor and β-arrestin-1, a scaffold protein with a well-established role in G protein-coupled receptor signaling. Using coimmunoprecipitation and in situ proximity ligation assays in A549 cells, we observed that β-arrestin-1 and unliganded GR interact in the cytoplasm and that, following glucocorticoid binding, the protein complex is found in the nucleus. We show that siRNA-mediated β-arrestin-1 knockdown alters GR protein turnover by up-regulating the E3 ubiquitin ligase Pellino-1, which catalyzesGRubiquitination and thereby marks the receptor for proteasomal degradation. The enhanced GR turnover observed in β-arrestin-1- deficient cells limits the duration of the glucocorticoid response on GR target genes. These results demonstrate thatβ-arrestin-1 is a crucial player for the stability of the glucocorticoid receptor. The GR/β-arrestin-1 interaction uncovered here may help unravel mechanisms that contribute to the cell type-specific activities of glucocorticoids. © 2019 American Society for Biochemistry and Molecular Biology Inc.. All rights reserved.


Cited by: 10; All Open Access, Green Open Access

Citation KeyPetrillo201911225
PubMed ID31167788